甜菜BvMARB基因全长cDNA的克隆和序列分析  

Gene Cloning and Sequence Analysis of the Full-length cDNA of Gene BvMARB from Beta vulgaris

作　　者：鲁振强[1,2] 潘彦遥 王锦霞[1,2] LU Zhen-qiang 1,2, PAN Yan-yao1,2, WANG Jin-xia1,2(1. Key Laboratory of Molecular Biology, College of Heilongjiang Province /College of Life Sciences, Heilongjiang University, Harbin150080, Heilongjiang; 2. Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, HeilongjiangUniversity, Harbin 150500, Heilongjian)

机构地区：[1]黑龙江省普通高等学校分子生物学重点实验室/黑龙江大学生命科学学院,哈尔滨150500 [2]黑龙江大学农业微生物技术教育部工程研究中心,哈尔滨150080

出　　处：《中国糖料》2018年第6期11-13,18,共4页Sugar Crops of China

基　　金：国家自然基金青年基金项目(31601229);黑龙江大学学科青年学术骨干百人支持计划项目;哈尔滨市科技局创新人才项目(2013RFQYJ025);黑龙江省高校基本科研业务费黑龙江大学专项资金项目(HDRCCX-201613);农业部糖料现代产业技术体系建设项目(CARS-170204;CARS-170111)

摘　　要：甜菜(Beta vulgaris)的M14品系在组织学、染色体遗传上表现出无融合生殖特征,使利用杂种优势获得优良种质成为可能。为了获得调控无融合生殖的候选基因,通过实验采用同源克隆及c DNA末端快速扩增(RACE)的方法,研究首次从甜菜M14品系中克隆了与生殖相关的基因BvMARB(Beta vulgaris matrix attachment regions binding protein)的全长cDNA序列。通过分析发现,其序列长度为2023 bp,开放阅读框为1 719 bp,编码572个氨基酸;蛋白分子量为63.17 KDa,等电点为9.2。同源比对表明,BvMARB与烟草MARB(GenBank No. AB059832)的相似度为67.20%,与番茄MARB(GenBank No. BT013761)的相似度为61.26%,与拟南芥MARB(GenBank No. AY096684)的相似度为57.19%。通过细胞内定位分析发现BvMARB所编码的蛋白主要存在于细胞核中。Sugar beet （Beta vulgaris） is an important sugar crop in China. Sugar beet （Beta vulgaris） line M14has an obvious characteristic of apomixes in histology and chromosome heredity. It is a kind of potential heterosisin high yield and elite quality inbred. In order to find out candidate genes for the regulation of apomixis, wecloned full-length cDNA sequence of BvMARB （Beta vulgaris matrix attachment regions binding protein） gene,which related to reproduction, and firstly used homologous clone method by Rapid Amplification of cDNA End（RACE）. The full-length cDNA sequence of BvMARB was 2 023 bp, and had an open reading frame of 1 719 bpencoding a polypeptide of 572 amino acids. The pI and protein molecular weight deduced from the amino acidssequence was 9.2 and 63.17 kDa, respectively. Homologous alignment indicates that it was 67.20% homologouswith tobacco （GenBank No. AB059832）, 61.26 % homologous with tomato （GenBank No. BT013761） and 57.19 %homologous with Arabdopsis （GenBank No. AY096684）. Prediction of protein localization sites （version6.4）indicated that the coding protein of BvMARB gene mainly existed in nucleus.

关 键 词：甜菜 BvMARB RACE 基因克隆 序列分析 

分 类 号：S566.3[农业科学—作物学]