高效液相色谱法测定甘蔗叶片中脱落酸  

Determination of Abscisic Acid in Sugarcane Leaves by High Performance Liquid Chromatography

作　　者：高欣欣[1] 刘少春[1] 刀静梅[1] 方志存[1] 邓军[1] 樊仙[1] GAO Xin-xin, LIU Shao-chun, DAO Jing-mei, FANG Zhi-cun, DENG Jun, FAN Xian(Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences/Yunnan Key Laboratory of Sugarcane Genetic Improvement,Kaiyuan 661699, Yunna)

机构地区：[1]云南省农业科学院甘蔗研究所/云南省甘蔗遗传改良重点实验室,云南开远661699

出　　处：《中国糖料》2018年第6期30-32,共3页Sugar Crops of China

基　　金：国家自然科学基金项目"甘蔗内源激素和主要酶系调控脱叶性的作用机理"(31160257)

摘　　要：建立了高效液相色谱(HPLC)法测定甘蔗叶片样品中内源ABA含量的分析方法。以流动相甲醇-5%乙酸为缓冲液,等度洗脱,紫外检测波长为254 nm。通过实验条件优化,0.25~10 mg/L范围内ABA标准溶液检测结果呈现良好的线性关系(相关系数r2=0.996),加标回收率为87.5%~102.4%,相对标准偏差不大于5%,检出限LOD=0.0202 mg/L,定量限LOQ=0.0675 mg/L。结果表明该方法能达到定量检测的目的,可以用于甘蔗叶片中内源ABA含量检测。High performance liquid chromatography （HPLC） method was used to test the ABA （Abscisic acid）content in sugarcane leaves. The sugarcane samples after grinding were extracted with methanol- acetic acid （5%）as mobile phases for isocratic elution. The detection wavelength was set at 254 nm with a UV-detector. With theoptimized conditions, the detection results of ABA standard solution in the range of 0. 25-10 mg/L showed a goodlinear relationship （Correlation coefficient r2=0.996）. The recoveries of samples reached 87.5%-102.4%, and therelative standard deviation was not more than 5%. The limit of detection （LOD） was 0.0202 mg/L, and the limit ofquantification （LOQ） was 0.0675 mg/L. The results showed that the method can achieve the purpose ofquantitative detection, which can be used to detect ABA content in sugarcane leaves.

关 键 词：甘蔗叶片 高效液相色谱 脱落酸含量 

分 类 号：S566.1[农业科学—作物学]