小RNA干扰沉默S100A13对甲状腺细胞侵袭、迁移的影响及机制  

Effect and mechanism of sliencing S100A4 by small RNA interference of S100A13 on thyroid cells

作　　者：李剑[1] 闫双通[1] 龚燕平[1] 卢艳慧[1] 孙般若[1] 成晓玲[1] 邵迎红[2] LI Jian;YAN ShuangtongI;GONG Yanping;LU Yanhui;SUN Banruo;CHENG Xiaoling;and SHAO Yinghong(Department of Geriatric Endocrinology;Outpatient Department,PLA General Hospital,Beijing 100853,China)

机构地区：[1]解放军总医院南楼内分泌科,北京100853 [2]解放军总医院门诊部,北京100853

出　　处：《武警医学》2018年第10期960-963,共4页Medical Journal of the Chinese People's Armed Police Force

摘　　要：目的探究干扰钙结合蛋白A13(calcium binding protein A13,S100A13)基因表达对甲状腺癌TPC-1细胞侵袭、迁移的影响及可能作用机制。方法采用小RNA干扰敲减甲状腺癌TPC-1细胞S100A13基因的表达,通过细胞划痕实验和Transwell迁移实验检测S100A13对TPC-1细胞迁移能力的影响,Transwell侵袭实验检测S100A13对TPC-1细胞侵袭能力的影响,免疫印迹试验(Western Blot)检测S100A13、基质金属蛋白酶2(MMP-2)、钙黏蛋白E(E-cadherin)、波形蛋白(Vimentin)蛋白的表达。结果与对照组相比,采用小RNA干扰的人甲状腺癌TPC-1细胞划痕宽度显著升高(25. 214±2. 687 vs 69. 223±5. 291),侵袭(0. 897±0. 082 vs 0. 521±0. 053)、迁移(1. 263±0. 252 vs 0. 759±0. 062)能力显著降低(P <0. 05),细胞中MMP-2(0. 523±0. 062 vs 0. 246±0. 033)、Vimentin (0. 863±0. 082 vs 0. 458±0. 053)蛋白表达显著降低(P <0. 05),E-cadherin(0. 445±0. 053 vs 0. 755±0. 078)蛋白表达显著增加(P <0. 05)。结论采用小RNA干扰S100A13表达可抑制甲状腺癌TPC-1细胞的侵袭、迁移,可能与其下调上皮间质转化和MMP-2的表达有关。Objective To investigate the effect of interference with the expression of calcium binding protein A13（ S100 A13）on the invasion and migration of thyroid carcinoma TPC-1 cells and the possible mechanism. Methods The expression of S100 A13 gene in thyroid cancer TPC-1 cells was knocked down by siRNA. The effect of S100 A13 on the migration of TPC-1 cells was evaluated with the wound healing scratch test and Transwell assay. The effect of S100 A13 on the invasiveness of TPC-1 cells was assessed by Transwell invasion test. The expressions of S100 A13,MMP-2,E-cadherin and vimentin were detected by Western Blot. Results Compared with the control group,the silencing of S100 A13 by siRNA in human thyroid carcinoma TPC-1 cells significantly increased the scratch width（ 25. 214 ± 2. 687 vs 69. 223 ± 5. 291）,but the ability of invasion（ 0. 897 ± 0. 082 vs 0. 521 ± 0. 053） and migration（ 1. 263 ± 0. 252 vs 0. 759 ± 0. 062） decreased significantly（ P〈0. 05）. The levels of MMP-2（ 0. 523 ± 0. 062 vs 0. 246 ± 0. 033） and vimentin（ 0. 863 ±0. 082 vs 0. 458 ± 0. 053） in S100 A13-treated cells were significantly decreased（ P〈0. 05）,while E-cadherin protein（ 0. 445 ± 0. 053 vs0. 755 ± 0. 078） was significantly increased（ P〈0. 05）. Conclusions Interference with S100 A13 expressions by siRNA inhibits the invasion and migration of thyroid carcinoma TPC-1 cells,which may be related to the inhibition of EMT and MMP-2.

关 键 词：甲状腺癌 S100A13 侵袭 迁移 

分 类 号：R736.1[医药卫生—肿瘤]