儋州鸡FSHβ基因的单核苷酸多态性研究  

Study on single nucleotide polymorphism of FSHβ gene in Danzhou chicken

作　　者：王鹏 侯德彧 施力光[2] 吴科榜 WANG Peng;HOU Deyu;SHI Liguang;WU Kebang(Institute of Tropical Agriculture and Forestry,Hainan University,Haikou 570228,China;Tropical Crops Germplasm Resources Institute,Chinese Academy of Tropical Agriculture Sciences,Danzhou 571737,China)

机构地区：[1]海南大学热带农林学院,海口570228 [2]中国热带农业科学院热带作物品种资源研究所,海南儋州571737

出　　处：《黑龙江畜牧兽医》2018年第19期19-22,239,共5页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家星火计划项目(2015GA800001);中国热带农业科学院基本科研业务费专项(1630032017038)

摘　　要：为了研究儋州鸡FSHβ基因单核苷酸多态性（SNP）,试验采用PCR产物直接测序法,对60份儋州鸡DNA样本的FSHβ基因进行PCR扩展和双向测序,对测序结果组装后使用DNAMAN软件进行比对,人工核对后筛查SNP位点。结果表明：PCR扩增均能得到与预期相符的清晰条带;测序结果与GenBank中登录的原鸡FSHβ序列比对显示,为儋州鸡FSHβ基因的片段;SNP位点筛查显示,儋州鸡FSHβ基因中含有25个SNP位点,即C-130T、A-332C、T-439C、G-591A、C-956A、G-1 233A、G-1 386C、G-1 466A、T-1 542C、T-1 543A、C-1 703T、C-1 714T、T-1 834C、A-1 867G、A-1 960C、C-2 021T、G-2 285T、G-2 399T、T-2 528C、A-2 536G、T-2 607C、G-2 770A、A-3 229G、-665处插入AA及-2 566处缺失C,这25个SNP位点包含了5个内含子突变和20个外显子突变,其中G-591A和G-1 233A为编码区错义突变。The aim of the present study was toinvestigate the single nucleotide polymorphisms（SNP） of the FSHβ gene in Danzhou chicken.PCR amplification and bidirectional sequencing of FSHβ gene from 60 Danzhou chicken DNA samples were performed by direct sequencing of PCR products. After the sequencing results were assembled, the DNAMAN software was used for comparison, and the SNP sites were screened by manual verification. The results showed that clear bands could be obtained by PCR amplification.The result of sequencing was compared with the sequence of FSHβ of chicken registered in GenBank, and the result showed that it was a fragment of FSHβ gene of Danzhou chicken.The screening of SNP loci showed that there were 25 SNP loci in the FSHβ gene of Danzhou chickens. They are C-130 T, A-332 C, T-439 C, G-591 A, C-956 A, G-1 233 A, G-1 386 C, G-1 466 A, T-1 542 C, T-1 543 A, C-1 703 T, C-1 714 T, T-1 834 C, A-1 867 G, A-1 960 C, C-2 021 T, G-2 285 T, G-2 399 T, T-2 528 C, A-2 536 G, T-2 607 C, G-2 770 A, A-3 229 G, insert AA at-665 site and deletion of C at-2 566 site.The 25 SNP loci contained 5 intron mutations and 20 exon mutations, of which G-591 A and G-1 233 A were missense mutations in the coding region.

关 键 词：儋州鸡 测序 FSHΒ SNP 编码区 

分 类 号：S858.31[农业科学—临床兽医学] Q75[农业科学—兽医学]