振腹推拿对原发性痛经大鼠模型的影响  

Impacts of abdominal vibration tuina therapy on primary dysmenorrheal in the rat models

作　　者：沈潜[1] 张巧娜 王康[1] 魏槃 王薇[2] 付国兵[1] 朱跃兰[1] SHEN Qian1, ZHANG Qiao- na1, WANG Kang1, WEI Pan1, WANG Wei2, FU Guo- bin1, ZHU Yue -lan1(1. Dongfang Hospital of Beijing University of Traditional Chinese Medicine, Beijing 100078 ;2. Wangjing Hospital, China Academy of Chinese Medical Sciences,Beijing 10010)

机构地区：[1]北京中医药大学东方医院,北京100078 [2]中国中医科学院望京医院,北京100102

出　　处：《世界中西医结合杂志》2018年第9期1252-1255,1309,共5页World Journal of Integrated Traditional and Western Medicine

基　　金：北京中医药大学中青年教师自主选题(2016-JYB-JSMS-053)

摘　　要：目的研究振腹推拿对原发性痛经模型大鼠的影响,基于前列腺素F_(2α)(PGF_(2α))、前列腺素E_2(PGE_2)及前列腺素F_(2α)受体(PTGFR)水平探讨振腹推拿治疗原发性痛经的作用机制。方法通过阴道涂片法筛选出动情周期规律的大鼠,随机分为空白组、模型组、阳性药物组、推拿组。采用苯甲酸雌二醇联合缩宫素制备原发性痛经大鼠模型。模型制备成功后,阳性药物组予以塞来昔布30 mg/(kg·d)的剂量灌胃治疗;推拿组采取振腹推拿治疗,15 min/只,均连续10 d。治疗结束后观察大鼠扭体反应,运用酶联免疫吸附测定、免疫组化等方法,检测大鼠血清及子宫组织中PGF_(2α)、PGE_2含量及子宫内膜上PTGFR的表达水平;观察各组大鼠子宫形态学变化。结果与模型组比较,推拿组大鼠的扭体潜伏时间延长,扭体次数减少(P<0.05);血清及子宫组织中PGF_(2α)含量均降低,PGE_2含量均升高(P<0.05);子宫内膜上PTGFR表达水平下降(P<0.05)。结论振腹推拿治疗原发性痛经效果显著,其作用机制可能是通过调节血清及子宫组织中PGF_(2α)、PGE_2的水平,抑制PTGFR在子宫内膜上的表达实现的。Objective To observe the effects of abdominal vibration tuina therapy on the model rats with primary dysmenorrheal and explore the effect mechanism based on the levels of PGF_（2α）,PGE_2 and PTGFR. Methods Through the vaginal smear screening method,the rats with the regular estrous cycle were collected and randomized into a blank group,a model group,a positive drug group and a tuina group. The estradiol benzoate and pitocin were used in combination to prepare the models of primary dysmenorrheal. After successful modeling,in the positive drug group,celecoxib was used for lavage treatment,30 mg/kg each day. In the tuina group,the abdominal vibration manipulation was used,15 min for each rat.The treatment was for 10 days consecutively. At the end of treatment,the body torsion reaction was observed in the rats. Using ELISA and IHC methods,the levels of PGF_（2α） and PGE_2 as well as the expression of PTGFR were determined in the serum and uterine tissue of the rats. The morphological change of the uterus was observed in the rats of each group. Results Compared with the model group,in the tuina group,the latent time of the body torsion was prolonged and the times of body torsion were reduced（ P〈0. 05）; the level of PGF_（2α） reduced and PGE_2 increased in the serum and uterine tissue（ P〈0. 05）; and the expression of PTGFR in the endometrium reduced（ P〈0. 05）. Conclusion The abdominal vibration tuina therapy achieves the significant effects on primary dysmenorrheal and its mechanism is probably through regulating the levels of PGF_（2α） and PGE_2 in the serum and uterine tissue and inhibiting the expression of PTGFR in the endometrium.

关 键 词：前列腺素E2 前列腺素F2Α 前列腺素PGF2α受体 原发性痛经 振腹推拿 

分 类 号：R271.113[医药卫生—中医妇科学] R-332[医药卫生—中医学]