载氧化铁PLGA微粒标记肌腱干细胞的磁共振／光声示踪的实验研究  

Dual-modal magnetic resonance and photoacoustic tracking of tendon stem cell labeled with PLGA/IO MPs

作　　者：成雪晴 胡紫玥 蒋京真 郝兰[3] 王志刚[3] 卢漫[1] Cheng Xueqing ;Hu Ziyue; Jiang Jingzhen; Hao Lan; Wang Zhigang; Lu Man(Ultrasound Medical Center, Sichuan Cancer Hospital, Chengdu 610041, China)

机构地区：[1]四川省肿瘤医院超声医学中心,成都610041 [2]川北医学院 [3]重庆医科大学超声影像学研究所

出　　处：《中华超声影像学杂志》2018年第10期905-910,共6页Chinese Journal of Ultrasonography

基　　金：国家自然科学基金（81571700）

摘　　要：目的探讨载氧化铁聚乳酸羟基乙酸微粒（PLGA／10MPs）标记肌腱干细胞（TSCs）的效率及磁共振（MR）／光声（PA）成像双模态体内示踪TSCs的可行性。方法利用前期制备的PLGA／IOMPs与前期实验分离培养的TSCs共同孵育以标记TSCs；于标记后3、7、14、21和28d对标记TSCs分别进行MR／PA成像及普鲁士蓝染色，应用电感耦合离子体原子发射光谱法（ICP—OES）测量标记TSCs细胞内Fe浓度。使用外科手术方式建立SD大鼠右侧肩袖损伤模型并移植标记TSCs，移植后第3、7、14、21和28d对大鼠肩袖进行MR／PA双模态成像观察标记TSCs。结果随着标记时间的延长，标记TSCs的MR和PA信号逐渐减弱，细胞内Fe含量逐渐减低，至标记后第28d细胞内Fe含量与未标记TSCs差异无统计学意义（1．45PgFe／cell对1．17PgFe／cell，P〉0．05）。大鼠肩袖损伤模型中，MR／PA成像能有效示踪标记TSCs分别长达21d和7d。结论PLGA／TOMPs能有效标记TSCs长达21d，双模态MR／PA成像在大鼠肩袖损伤模型中能有效示踪标记TSCs。Objective To detect the efficiency of the newly developed PLGA/IO MPs in tracking tendon stem cells （TSCs） by magnetic resonance （MR） and photoacoustic （PA） imaging. Methods Both PLAG/IO MPs and TSCs were prepared and acquired according to the previous study, and TSCs were incubated with PLGA/IO MPs for labeling. TSCs were collected for MR and PA imaging, prussian blue staining was performed, and the iron concentration of labeled TSCs was determined using inductively coupled plasma optical emission spectrometry （ICP-OES） at 3, 7, 14, 21 and 28 days after labeling respectively. The rotator cuff injury model was built on the right side of SD rats by surgery and the labeled TSCs were implanted instantly. Dual-modal MR/PA imaging was performed to observe the implanted labeled TSCs at day 3,7, 14,21 and 28 after implantation respectively. Results Along with the increase of labeling time, both MR and PA signal of labeled TSCs decreased gradually, and the amount of intraeellular Fe loading was gradually decreased. At day 28, the difference of Fe concentration per cell between labeled TSCs and non labeled TSCs was not significant （1.45 pg Fe/cell vs 1.17 pg Fe/cell, P 〉0.05）. MR and PA imaging allowed a long-term tracking of labeled TSCs for 21 and 7 days respectively in the rat rotator cuff injury model. Conclusions PLGA/IO MPs are able to label TSCs for up to 21 days, and dual-modal MR/PA imaging could be used to track the labeled TSCs in the rat rotator cuff injury model.

关 键 词：磁共振成像 光声成像 肌腱干细胞 细胞示踪 

分 类 号：R445.2[医药卫生—影像医学与核医学]