候选无细胞百白破-Sabin株灭活脊髓灰质炎联合疫苗免疫小鼠的效果研究  

Immunogenicity of a candidate diphtheria, tetanus, acellular pertussis and Sabin strain inactivated poliovirus combined vaccine in a mouse model

作　　者：江文文 梁疆莉[1] 高娜[1] 顾琴[1] 马艳[1] 牟大超 史荔[1] 孙明波[1] Jiang Wenwen;Liang Jiangli;Gao Na;Gu Qin;Ma Yah;Mou Dachao;Shi Li;Sun Mingbo(Institute of Medical Biology of Chinese Academy of Medical Science ＆ Peking Union Medical College,Yunnan Key Laboratory of Vaccine Research ＆ Develop-ment on Severe Infectious Diseases,Kunming 650118,Yunnan,China)

机构地区：[1]中国医学科学院北京协和医学院医学生物学研究所云南省重大传染病疫苗研发重点实验室

出　　处：《中国疫苗和免疫》2018年第5期559-564,共6页Chinese Journal of Vaccines and Immunization

基　　金：国家科技重大专项课题(2015ZX09101031);中国医学科学院重大协同创新项目(2016-I2M-1-019)

摘　　要：目的观察候选无细胞百白破-Sabin株灭活脊髓灰质炎联合疫苗（DTaP-sIPV）在小鼠中的免疫原性,为疫苗临床前研究提供依据。方法将候选疫苗分别稀释成5倍、10倍、20倍三组,以相应稀释的无细胞百白破-灭活脊髓灰质炎-b型流感嗜血杆菌联合疫苗（DTaP-IPV/Hib）、无细胞百白破联合疫苗（DTaP）为对照组,每组按0、4、8周3剂免疫程序免疫NIH小鼠（10只/组）,检测各组小鼠每剂免疫后28d的血清百白破组分抗体水平。结果候选疫苗三个组完成3剂次免疫后PT抗体的几何平均浓度（GMC）（IU/mL）分别为2 157.29、2 142.51、2 135.17;FHA抗体GMC分别为10 098.30、10 068.33、9 143.30;PRN抗体GMC分别为1 683.86、1 130.29、193.00;DT抗体几何平均滴度（GMT）（1∶）分别为23.48、22.44、22.44;TT抗体GMT分别为22.31、21.24、21.34。候选疫苗三个组PT、FHA、DT、TT抗体水平均高于两种疫苗对照组或者与其无显著性差异,但PRN抗体水平低于DTaP对照组。结论候选疫苗免疫小鼠具有良好的免疫原性。Objective To assess immunogenicity of a candidate diphtheria, tetanus, acellular pertussis and Sabin strain inactivated poliovirus combined vaccine（DTaP-sIPV） in a mouse model in anticipation of further preclinical research. Methods We divided the candidate vaccine into 5-, 10-and 20-fold dilution groups and selected corresponding dilution groups of diphtheria, tetanus, acellular pertussis, inactivated poliovirus and Haemophilus influenzae type b combined vaccine（DTaP-IPV/Hib）, and diphtheria, tetanus and acellular pertussis combined vaccine（DTaP） as controls. We vaccinated 10 NIH mice in each group at 0, 4 and 8 weeks for testing serum antibodies to diphtheria, tetanus and pertussis antigens 28 days after each dose. Results Among the three candidate vaccine groups, geometric mean concentrations（GMCs）（IU/mL） of antibody for anti-PT after vaccination, respectively, were 2 157.29, 2 142.51, and 2 135.17; GMCs for anti-FHA were 10 098.30, 10 068.33, and 9 143.30; GMCs for anti-PRN were 1 683.86, 1 130.29, and 193.00; geometric mean titers（GMTs）（1∶） for anti-DT were 23.48, 22.44, and 22.44; GMTs for anti-TT were 22.31, 21.24, and 21.34. Antibody levels for anti-PT, anti-FHA, anti-DT and anti-TT were higher among candidate vaccine groups than among control vaccine groups or were not significantly different by group. Antibody levels for anti-PRN were lower among candidate vaccine groups than among DTaP control groups. Conclusions The candidate vaccine had good immunogenicity in the mouse model.

关 键 词：无细胞百白破-Sabin株脊髓灰质炎联合疫苗 小鼠 免疫原性 

分 类 号：R186[医药卫生—流行病学]