网织红细胞参数在新生儿母婴ABO血型不合溶血诊断中的临床价值  

Clinical value of reticulocyte parameters in newborn hemolytic diagnosis of ABO blood group incompatibility between mother and infant

作　　者：杨娟[1] Yang Juan(Department of Laboratory,Hunan Chirldren＇s Hospital,Changsha 410007,Hunan,China)

机构地区：[1]湖南省儿童医院检验中心,湖南长沙410007

出　　处：《实用检验医师杂志》2018年第3期173-175,共3页Chinese Journal of Clinical Pathologist

摘　　要：目的探讨网织红细胞参数在新生儿母婴ABO血型不合溶血病诊断中的临床价值。方法选择2017年1月—2018年1月湖南省儿童医院收治的100例黄疸新生儿作为研究对象,根据是否发生新生儿母婴ABO血型不合溶血将其分为溶血病组(55例)和非溶血病组(45例)。采用流式细胞术和自动荧光染色技术,检测所有患儿网织红细胞参数[包括未成熟网织红细胞指数(IRF)、低荧光网织红细胞百分比(LFR)、中荧光网织红细胞百分比(MFR)、高荧光网织红细胞百分比(HFR)、网织红细胞百分比(RET)],对比两组患儿的检测结果。绘制受试者工作特征曲线(ROC),分析各网织红细胞参数对新生儿母婴ABO血型不合溶血病的诊断价值。结果与非溶血病组比较,溶血病组IRF、MFR、HFR、RET均明显升高[IRF:(38.94±5.26)%比(27.69±6.75)%,MFR:(22.38±3.47)%比(17.34±3.25)%,HFR:(16.29±4.15)%比(10.39±5.78)%,RET:(9.52±3.15)%比(4.26±1.69)%,均P<0.05],LFR明显降低[(60.63±5.19)%比(71.32±6.49),P<0.05]。ROC曲线分析显示,IRF、LFR、MFR、HFR和RET诊断新生儿母婴ABO血型不合溶血病的敏感度分别为83.6%、65.4%、73.2%、84.1%、85.2%,特异度分别为94.3%、58.9%、62.8%、95.4%、96.5%。结论在新生儿母婴ABO血型不合溶血病诊断中,应用新型网织红细胞参数能够提高新生儿母婴ABO血型不合溶血病诊断的准确率,具有显著的应用价值,可将其应用于临床疾病的诊断中。Objective To investigate the clinical diagnostic value of reticulocyte parameters in newborn hemolytic disease due to ABO blood type incompatibility between mother and infant. Methods One hundred newborns with jaundice in Children＇s Hospital of Hunan Province from January 2017 to January 2018 were selected as the research subjects, and according to the presence or absence of neonate hemolysis due to ABO blood type incompatibility between mother and infant, they were divided into a hemolytic disease group（55 cases） and a non hemolytic disease group（45 cases）. The flow cytometry and automatic fluorescence staining technique were used to measure the parameters of reticulocyte [including immature reticulocyte index（IRF）, low fluorescence reticulocyte percentage（LFR）, middle fluorescence of reticulocyte percentage（MFR）, high fluorescent reticulocyte percentage（HFR）, reticulocyte percentage（RET）] for all neonates, and the test results were compared between the two groups. The receiver operating characteristic curve（ROC） was drawn to analyze the diagnostic value of reticulocyte parameters on neonatal hemolytic disease due to ABO blood type incompatibility between mother and infant. Results Compared with the non hemolytic disease group, the IRF, MFR, HFR and RET in hemolytic disease group were all higher significantly [IRF：（38.94±5.26）% vs.（27.69±6.75）%, MFR：（22.38±3.47）% vs.（17.34±3.25）%, HFR：（16.29±4.15）% vs.（10.39±5.78）%, RET：（9.52±3.15）% vs.（4.26±1.69）%, all P〈0.05], while LFR was significantly lower in hemolytic group [（60.63±5.19）% vs.（71.32±6.49）%, P〈0.05]. ROC analysis showed that the sensitivity of IRF, LFR, MFR, HFR and RET was 83.6%, 65.4%, 73.2%, 84.1%, 85.2%, respectively, and the specificity was 94.3%, 58.9%, 62.8%, 95.4%, 96.5%, respectively in diagnosing hemolytic disease due to ABO blood type incompatibility between mother and infant. Conclusion In the diagnosis of neonatal hemolytic disease caused by incompat

关 键 词：网织红细胞 新生儿 ABO血型不合 溶血 诊断 

分 类 号：R722.18[医药卫生—儿科]