黑香蕉葡萄不同器官发生途径再生体系的优化  

Optimization of Vitis vinifera×V. labrusca ‘Hei Xiangjiao'adventitious buds regeneration via organogenesis

作　　者：常新 梁晋军 刘振华 肖坤 王燕 崔海洋 胡建芳 CHANG Xin;LIANG Jinjun;LIU Zhenhua;XlAO Kun;WANG Yan;CUI Haiyang;HU Jianfang(College of Horticulture,China Agricultrual University,Beijing 100093,China)

机构地区：[1]中国农业大学园艺学院,北京100193

出　　处：《中国农业大学学报》2018年第9期43-52,共10页Journal of China Agricultural University

基　　金：国家自然科学基金项目(31471842);国家重点研发计划(SQ2018YFD020082)

摘　　要：为探讨葡萄高效再生体系的优化,以黑香蕉葡萄品种叶片、叶柄、茎尖分生组织为外植体,对其再生体系进行了优化,同时比较了不同器官再生的效果。结果显示:适合叶片再生的培养基为:MS+2.0mg/L 6-BA+0.1mg/L IBA,但再生率仅为4.88%;适合叶柄再生的培养基为:NN69+1.33mg/L6-BA+0.02mg/L NAA,再生率为16.92%。说明叶柄的再生率高于叶片但两者都没有超过20%。并且NAA对黑香蕉叶柄的再生效果好于IBA。NN69基本培养基要优于MS。以茎尖为外植体在起始培养基MS+6-BA 1.40mg/L+NAA 0.09mg/L和诱导培养基培养MS+2mg/L 6-BA+0.09mg/L NAA上通过反复损伤芽尖得到茎尖分生组织(Meristematic bulk,MB),其不定芽诱导率高达87.63%。说明利用茎尖分生组织建立的葡萄再生体系更适合葡萄的遗传转化。To optimize the regeneration system of grape,the regeneration system of Vitis vinifera×V.labrusca ‘Hei Xiangjiao＇leaf,petiole and shoot apical meristem were optimized,and the regeneration effects of different organs were compared.The results showed that：The optimum media for the regeneration of adventitious buds from leaf is MS ＋2.0 mg/L 6-BA ＋ 0.1 mg/L IBA,but the regeneration rate is only 4.88%;The suitable medium for petiole is NN69＋ 1.33 mg/L 6-BA＋ 0.02 mg/L NAA,the regeneration rate was increased to 16.92%;The regeneration rate of petiole was higher than that of leaf,but neither exceeded 20%;The effects of NAA enhanced the regeneration of adventitious buds from petioles better than that of IBA;The NN69 medium was better than MS,and the regenerated rate at 40 days was maximum;The stem was cultured on initiation media（MS＋6-BA 1.40 mg/L＋NAA 0.09 mg/L）and then subcultured on induction medium（MS＋ 6-BA 2 mg/L＋ NAA 0.09 mg/L）and wounding treatments was supplied to produced meristematic bulk tissue.The adventitious bud induction rate is 87.63%.The results indicated that the regeneration system of meristematic bulk was more suitable for grape genetic transformation.

关 键 词：葡萄 器官发生 培养基 外植体 分生体 

分 类 号：S513[农业科学—作物学]