维持培养基组分对流感病毒增殖效果的影响  

Effect of maintenance medium components on the propagation efficiency of influenza virus

作　　者：黄世腾[1] 杨瑞军[1] 吕磊[1] HUANG Shi-teng,YANG Rui-jun,LYU Lei(Quzhou Municipal Center for Disease Control and Prevention, Quzhou, Zhejiang 324000, China)

机构地区：[1]衢州市疾病预防控制中心,浙江衢州324000

出　　处：《中国公共卫生管理》2018年第5期613-616,共4页Chinese Journal of Public Health Management

摘　　要：目的探究维持培养基不同组分对流感病毒增殖的影响,提高病毒增殖效率。方法通过改变维持培养基的组分,分别添加不同浓度的TPCK-胰酶、谷氨酰胺、葡萄糖和丁酸钠,用其对流感病毒在MDCK细胞中进行增殖培养,在24h、48h、72h、96h对培养物进行血凝素(HA)滴度测定;同时检测不同浓度的丁酸纳对MDCK细胞活性的影响。结果流感病毒新甲型H1N1、A(H3N2)和B(Victoria)增殖的最佳胰酶浓度为3μg/m L,B(Yamagata)最佳胰酶浓度为2μg/m L;各亚型流感病毒增殖的最适宜谷氨酰胺浓度为4mM;最适宜葡萄糖浓度为(20～30) m M;最佳丁酸钠浓度为0. 5mM,过高的丁酸钠浓度对流感病毒增殖有抑制作用,丁酸钠降低细胞活性的能力随浓度提高而增加。结论调节维持培养基组分浓度能够提高流感病毒的增殖效率,为高效地进行流感病毒分离和流感疫苗生产提供基础数据。Objective To investigate the effects of maintenance medium components on the propagation efficiency of influenza virus,improve the efficiency of virus proliferation. Methods The concentrations of medium components were changed,included TPCK-trypsin,glutamine,glucose and sodium butyrate. The influenza virus were cultivated on MDCK cell in different medium,and then hemagglutination titers were detected at 24 h,48,72 h and 96 h. Meanwhile,the effect of different concentrations of sodium butyrate on the activity of MDCK cell was investigated. Results The optimal concentration of TPCK-trypsin were 3μg/ml（ H1 N1,H3 N2 and Victoria） and 2μg/ml（ Yamagata）. The different subtypes of influenza virus had the most suitable concentration of glutamine of 4 mM,the most suitable concentration of glucose was 20 ～ 30 m M,and the most suitable concentration of sodium butyrate was 0. 5 mM. The propagation of influenza virus were inhibited by high concentration of sodium butyrate. The cell activity was lower with increasing concentration of sodium butyrate. Conclusion The propagation efficiency of influenza virus increased by adjusting the concentration of different medium components,in order to provide guidance for isolation of influenza virus and vaccine production.

关 键 词：流感病毒 病毒增殖 MDCK细胞 TPCK-胰酶 丁酸钠 

分 类 号：R446.5[医药卫生—诊断学]