精液与附睾结节穿刺标本结核杆菌DNA检测在附睾结核诊断中的价值  

The value of tubercle bacilli DNA detection of seminal fluid and epididymal nodules puncture specimen in epididymal tuberculosis diagnosis

作　　者：左建强[1] 包建成[1] 王惠泉[1] 杨跃军[1] 郭子臣[1] 徐伟民[1] 冯长梅[2] ZUO Jianqiang;BAO Jiancheng;WANG Huiquan;YANG Yuejun;GUO Zichen;XU Weimin;FENG Changmei(Department of Urinary Surgery,The Third Affiliated Hospital of Baotou Medical College,Baotou 014030,China;Department of Clinical Lab,The Third Affiliated Hospital of Baotou Medical College,Baotou 014030,China)

机构地区：[1]包头医学院第三附属医院泌尿外科,包头014030 [2]包头医学院第三附属医院检验科,包头014030

出　　处：《外科研究与新技术》2018年第3期157-159,共3页Surgical Research and New Technique

基　　金：包头市医药卫生科技发展基金计划项目(2013052)

摘　　要：目的探讨精液及附睾结节穿刺标本中结核杆菌DNA含量检测在附睾结核诊断中的价值。方法对2010年1月—2018年2月诊治的42例附睾结节患者,均行附睾结节穿刺术及留取精液,将标本分为两份分别行结核杆菌DNA检测及涂片镜检结核杆菌,计算结核杆菌阳性率。结果结核杆菌DNA检测精液标本阳性率28. 57%、附睾穿刺标本阳性率42. 86%、组织标本高于精液标本P <0. 01;精液标本与附睾穿刺标本结核杆菌涂片检测阳性率比较无统计学差异(P> 0. 05)。结论对可疑附睾结核患者的精液及附睾结节穿刺应用实时荧光定量PCR方法检测结核杆菌DNA含量,是一种有价值的诊断手段。Objective To assess the value of tubercle bacilli DNA detection of seminal fluid and epididymal tuberculous puncture specimen in epididymal tuberculosis diagnosis. Methods From Jan. 2010 to Feb. 2018, 42 patients with epididymal nodules were selected. Tubercle bacilli DNA detection of seminal fluid and epididymal nodules puncture specimen were taken. Tubercle bacilli were also tested and positive tuberculosis rate was calculatel. Results The positive rate of TB-DNA in the epididymal puncture group was significantly higher than that in the semiral fluid group （42.86% vs. 28.57%, P 〈 0.01 ）. The positive rates of smear test had no significant difference between the two groups （ P 〉 0.05）. Conclusion Tubercle bacilli DNA detection of seminal fluid and epididymal nodules puncture specimen have a great value in diagnosing epididymal tuberculosis.

关 键 词：精液 附睾穿刺 结核杆菌DNA检测 附睾结核 

分 类 号：R447[医药卫生—诊断学]