pH敏感型氯霉素眼用原位凝胶的制备及释放度考察  

Preparation and in vitro release of chloramphenicol in situ pH-sensitive gel

作　　者：赵玉娜[1] 郝秀娟[1] ZHAO Yu′na;HAO Xiujuan(General Hospital of Ningxia Medical University,Yinchuan 750004,China)

机构地区：[1]宁夏医科大学总医院,银川750004

出　　处：《西北药学杂志》2018年第6期793-796,共4页Northwest Pharmaceutical Journal

基　　金：宁夏医科大学青年基金项目(编号:XM2015098)

摘　　要：目的制备具有pH敏感型的氯霉素眼用原位凝胶,并对其凝胶特性及体外释放进行考察。方法以卡波姆为凝胶基质,羟丙甲基纤维素为增稠剂,以溶液的黏度、凝胶形成能力等为评价指标,确定制备处方和工艺。采用紫外联立方程新解法测定凝胶中氯霉素的含量,进行药物体外释放度研究。结果卡波姆用量为3g·L-1,HPMC用量为10g·L-1时,制备的氯霉素眼用凝胶非生理条件下呈流动状态,生理条件下形成较强的凝胶,且其在人工泪液中滞留时间长。体外释放结果显示,其释药平缓,具有较好的缓释特征。结论优选处方工艺稳定,含量测定方法可靠,适用于pH敏感型氯霉素眼用原位凝胶的制备和评价。Objective To prepare chloramphenicol insitu pH-sensitive gel and to study the drug release in vitro . Methods Carbopol was used as pH sensitive base material and hydroxypropyl methyl cellulose （HPMC） as thickening agent.The formulation was selected according to the viscosity and the in situ gelling capacity.The concentration of chloramphenicol was determined directly by violet spectrometry,then the drug release in vitro was determined. Results The optimum concentrations of Carbopol and HPMC for chloramphenicol in situ pH-sensitive gel were 3 and 10 g·L -1 ,respectively.The prepared gel was liquid at non-physiological condition and turned into a strong gel under physiological conditions.And it showed a long residence time in the artificial tears.The gel showed good sustained release characteristics in vitro . Conclusion The optimized formulation has a good reproducibility and can be used for the preparation of chloramphenicol insitu pH-sensitive gel.

关 键 词：氯霉素 PH敏感 眼用原位凝胶 卡波姆 体外释放 

分 类 号：R94[医药卫生—药剂学]