塔里木盆地5个生态小区稀有放线菌分离及合成抗生素基因分布  

Isolation of Rare Actinobacteria in 5 Ecodistricts of Tarim Basin and Distribution of the Genes Synthesizing Antibiotics

作　　者：朱荣贵[1] 关统伟 姜秀娟 ZHU Rong-gui;GUAN Tong-wei;JIANGXiu-juan(College ofBioertgineering,Sichuan University of Science Engineering,Zigong 643000;College of Food Science and Biological Engineering,Xihua University,Chengdu 610039)

机构地区：[1]四川理工学院生物工程学院,自贡643000 [2]西华大学食品与生物工程学院,成都610039

出　　处：《生物技术通报》2018年第9期230-236,共7页Biotechnology Bulletin

基　　金：国家"973计划"前期研究专项(2010CB134505);国家自然科学基金项目(31060001);四川省教育厅项目(16ZB0244);校级人才引进项目(2015RC04)

摘　　要：为调查塔里木盆地5个代表性生态小区的稀有放线菌分布,并评估它们产生抗生素类活性物质的潜力。采集塔里木盆地5个生态小区混合土样5份,采用7种培养基分离样品中的稀有放线菌。通过检测分析I型PKS、Ⅱ型PKS、NRPS、APH、HMG-CoA五种抗生素合成相关基因的分布,评估该地区稀有放线菌产生抗生素的潜力。结果表明:(1)基于分子鉴定,经合并重复,共分离得到18种稀有放线菌,属于放线菌的10个属。(2)塔里木河河岸林生态小区土样稀有放线菌分离种类最多(10种),为8个属,塔克拉玛干沙漠塔里木河东部生态小区最少(4种),为4个属。(3)18株稀有放线菌中有9株含有I型PKS基因、4株菌含有Ⅱ型PKS基因、4株菌含有APH基因、3株菌含有NRPS基因,有一株链孢囊菌同时含有I型PKS、Ⅱ型PKS、NRPS、APH 4种基因。5个生态小区稀有放线菌种类较多,并含有较为丰富的与抗生素合成相关的基因。This work is for investigating the distribution of rare actinobacteria in different ecodistricts and assessing the potential of antibiotic production of these actinobacteria. Total 5 soil samples were collected from the Tarim Basin,and rare actinobacteria were isolatedfrom these samples by 7 media. And then the potential of antibiotic productions was assessed by detecting type I PKS,type Ⅱ PKS,NRPS,APH and HMG-CoA genes,related to antibiotics synthesis. The results showed that ：（1）by molecular identification,we obtained 18 kindsof rare actinobacteria strains belonging to 10 different genera;（2）the riparian forest ecodistrict in the Tarim Basin had the most species of rareactinobacteria,and there were 8 genera;while the least one was the east of the Talimu river ecodistrict in Taklimakan desert,and there were4 genera;（3）9 of these 18 strains had type I PKS gene,4 strains had type Ⅱ PKS gene and APH gene,and 3 strains had NRPS gene;therewas one Streptosporangium strain that contained type I PKS gene,type Ⅱ PKS gene,NRPS gene and APH gene. Conclusively,5 ecological zones are found to have relatively more rare actinomycetes which also contain abundant genes in associated with the synthesis of antibiotics.

关 键 词：塔里木盆地 生态小区 稀有放线菌 功能基因筛选 

分 类 号：Q93[生物学—微生物学]