植物来源内生真菌Bipolaris sp.TJ-1的次生代谢产物研究  

Secondary metabolites of a plant-derived endophytic fungus Bipolaris sp.TJ-1

作　　者：黄传俊[1,2] 刘梦婷 胡正喜[3] 张勇慧[3] HUANG Chuan-jun;LIU Meng-ting;HU Zheng-xi;ZHANG Yong-hui(Hubei Provincial Hospital of Tra ditional Chinese Medicine,Hubei Wuhan 430061,China;Hubei Academy of Traditional Chinese Medicine and Pharmacy,Hubei Wuhan 430074,China;Hubei Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation,School of Pharmacy,Tongji Medical College,Huazhong University of Science and Technology,Hubei Wuhan 430030,China)

机构地区：[1]湖北省中医院,湖北武汉430061 [2]湖北省中医药研究院,湖北武汉430074 [3]华中科技大学同济医学院药学院湖北省天然药物化学与资源评价重点实验室,湖北武汉430030

出　　处：《中国医院药学杂志》2018年第18期1907-1910,共4页Chinese Journal of Hospital Pharmacy

基　　金：教育部长江学者奖励计划(编号:T2016088);国家杰出青年科学基金(编号:81725021)

摘　　要：目的:研究植物内生真菌Bipolaris sp.TJ-1的次生代谢产物。方法:利用硅胶、凝胶Sephadex LH-20、高效液相等色谱方法,对菌种的大米发酵提取物进行系统的分离纯化,并根据波谱学数据和理化常数分析等手段鉴定化合物结构。结果:共分离鉴定9个化合物,分别为penicillide(1),dehydroisopenicillide(2),3,4-二甲氧基肉桂酸(3),N-苯乙基乙酰胺(4),(2R,4R)-harzialactone A(5),4-hydroxykigelin(6),6-methoxymellein(7),3,4-dihydro-6,8-dihydroxy-3-methylisocoumarin(8)和4-hydroxy-6-methoxymellein(9)。结论:化合物1~9均为首次从离蠕孢属内生真菌中分离得到。OBJECTIVE To study the secondary metabolites of a plant-derived endophytic fungus Bipolaris sp.TJ-1.METHODS Various column chromatography methods,such as silica gel,Sephadex LH-20,and semi-preparative HPLC,were applied to isolate and purify the secondary metabolites of fermented rice medium of the fungus Bipolaris sp.,and their chemical structures were identified via NMR data and physical chemical constants.RESULTS Nine compounds were isolated and identified as penicillide（1）,dehydroisopenicillide（2）,3,4-dimethoxycinnamic acid（3）,N-phenethylacetamide（4）,（2 R,4 R）-harzialactone A（5）,4-hydroxykigelin（6）,6-methoxymellein（7）,3,4-dihydro-6,8-dihydroxy-3-methylisocoumarin（8）,and 4-hydroxy-6-methoxymellein（9）,respectively.CONCLUSION Compounds 1-9 are isolated and identified from the genus Bipolaris for the first time.

关 键 词：腺梗豨莶草 内生真菌 离蠕孢属 次生代谢产物 

分 类 号：R285[医药卫生—中药学]