重组蛋白下游连续纯化技术的研究进展  

The Development of Downstream Continuous Purification Technology of Recombinant Protein

作　　者：赵伟 李敬达[1] 刘庆平[1] ZHAO Wei;LI Jing-da;LIU Qing-ping(Key Laboratory of Lipid Metabolism in Liaoning Province,College of Life Science and Technology,Dalian University,Dalian 116622,China)

机构地区：[1]辽宁省糖脂代谢重点实验室大连大学生命科学与技术学院

出　　处：《中国生物工程杂志》2018年第10期181-188,共8页China Biotechnology

基　　金：国家自然科学基金(81270361)资助项目

摘　　要：重组蛋白质的纯化一直是限制其生产的瓶颈环节,传统纯化工艺以批次模式为主,普遍存在成本高,处理效率及回收率低等问题。近年来,随着连续离心,过滤,层析技术的开发和应用,传统工艺的缺陷得到有效的克服。主要以下游纯化工艺为主线,对工业大规模纯化过程中所涉及的连续技术的特点及应用进行了归纳分析。对纯化工艺中的核心技术-层析,以及未来重组蛋白质的纯化技术进行了展望,旨在为重组蛋白质生产工艺的优化提供新的理论依据和指导思想。Purification of recombinant proteins has always been a bottleneck limiting its production. The traditional purification process is mostly batch based mode,which is characterized by high cost,low treatment efficiency and low recovery. Recently,with the development and application of continuous centrifugation,filtration,chromatography,and technologies,they have effectively overcome the defects of traditional processes.Based on downstream purification processes,the characteristics and applications of continuous technologies involved in large-scale industrial purification are summarized and analyzed. The core technology in the purification process-chromatography,and the prospects for the future technology of recombinant proteins purification are prospected,which is aimed at providing new theoretical evidences and guidance for optimization of biopharmaceutical production process.

关 键 词：连续纯化技术 工业生产 蛋白纯化 层析 

分 类 号：Q819[生物学—生物工程]