枳壳趁鲜切制工艺优选及药效研究  

Optimization of fresh-cuting process and its pharmacodynamics of Aurantii Fructus

作　　者：罗雪晴 张金莲[1] 颜冬梅[1] 钟凌云[1] 李娜[1] 欧阳辉[1] 夏澜婷 祝婧[1] 罗时昌 LUO Xue-qing;ZHANG Jin-lian;YAN Dong-mei;ZHONG Ling-yun;LI Na;OUYANG Hui;XIA Lan-ting;ZHU Jing;LUO Shi-chang(Jiangxi University of Traditional Chinese Medicine,Nanchang 330004,China)

机构地区：[1]江西中医药大学,江西南昌330004

出　　处：《中草药》2018年第20期4743-4747,共5页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(81560651);国家中医药管理局公益性科研专项(201507002-2);江西省卫生计生委课题(2016A011)

摘　　要：目的研究不同切制时间、干燥方法对枳壳趁鲜切制饮片的药典指标成分的影响,比较优选趁鲜切制工艺饮片与传统饮片成分和药效的差异。方法采用Diamonsil C18色谱柱(250 mm×4.6 mm,5μm),流动相为乙腈-磷酸水(20∶80,p H3.0),体积流量1.0 m L/min,柱温30℃,检测波长283 nm,进样量20μL,对不同工艺枳壳趁鲜切制饮片的柚皮苷和新橙皮苷的含量进行测定,优选趁鲜切制的时间及干燥方法,观察优选趁鲜切制工艺饮片与传统饮片对小鼠肠推进率、胃排空率、胃泌素等的影响。结果同一产地枳壳趁鲜切制晒干品指标成分柚皮苷和新橙皮苷含量高于相应天数的60℃烘干品,晾晒3 d切制晒干的饮片含量最高,优选的趁鲜切制工艺饮片在肠推进率、胃排空率、胃泌素指标上与传统饮片差异不显著(P>0.05),血液流变性指标差异显著(P<0.05)。结论优选的枳壳趁鲜切制工艺为鲜枳壳横向对半切,晾晒3 d,再切薄片,晒干。所得趁鲜切制饮片与传统饮片在胃肠动力学药效指标上作用强度相当。Objective To study the effect of different cutting time and drying methods on the index components of pharmacopeia in different fresh-cutting Aurantii Fructus,and investigate the difference of component and efficacy between the preferred fresh-cutting Aurantii Fructus and the traditional slices.Methods The HPLC system consisted of a Diamonsil C18（250 mm × 4.6 mm,5 μm） with acetonitrile/phosphoric acid water（20∶80,p H 3.0） as the mobile phase,the flow rate was 1.0 m L/min,column temperature was 30 ℃,detection wavelength was 283 nm,and the injection volume was 20 μL.The content of naringin and neohesperidin in fresh-cutting Aurantii Fructus with different processing methods was determined to optimize the cutting time and drying method.At the same time,the effects of sclices from fresh-cutting and traditional process on the small intestine propulsion rate,gastric emptying rate,gastrin,and hemorheology were determined.Results The content of naringin and neohesperidin of the fresh-cutting Aurantii Fructus that dried in sun was higher than that dried at 60 ℃ in oven at the corresponding days from the same origin,and the highest content was from fresh-cutting slices dried in sun for 3 d.There was no significant difference in the indexes of small intestine propulsion rate,gastric emptying rate,and gastrin between the preferred fresh-cuting Aurantii Fructus and the traditional ones,but the indexes of hemorheology showed significant difference（P 0.05）.Conclusion The preferred fresh-cutting Aurantii Fructus（processed by half cutting horizontally,drying for 3 d,and then cutting into thin slices and drying in the sun） showes no significant difference in gastrointestinal motility indexes when compared with the traditional ones.

关 键 词：枳壳 趁鲜切制 切制时间 干燥方法 柚皮苷 新橙皮苷 肠推进 胃排空 胃泌素 血液流变学 

分 类 号：R285.5[医药卫生—中药学]