IL-17A对HepG2细胞凋亡的影响及其机制  

Effect and Novel Mechanism of IL-ITA on Apoptosis of HepG2 Cell

作　　者：尹文君[1] 李绵利 王秋平 姜孝新[1] YIN Wen-jun;LIMian-li;WANG Qiu-ping(Department of Clinical Laboratory,the First Affiliated Hospital to University of South China,Hengyang,Hunan 421001,China)

机构地区：[1]南华大学第一附属医院检验科,湖南衡阳421001 [2]船山实验中学,湖南衡阳421001

出　　处：《医学临床研究》2018年第10期1883-1885,共3页Journal of Clinical Research

基　　金：湖南省自然科学基金资助项目（编号：2018JJ3166）

摘　　要：【目的】探讨白介素-17A（IL-17A）对HepG2细胞凋亡的影响及作用机制。【方法】培养正常肝细胞（L-02）及肝癌细胞（Huh7、HepG2和PLC／PRF／5），荧光实时定量PCR（qRT-PCR）、Westernblot测定其细胞IL-17A表达。然后取HepG2细胞，予重组IL-17A（rIL-17A）和（或）髓细胞白血病因子-1（Mel-1）siRNA处理，流式细胞术分析细胞凋亡率；qRT-PCR、Westernblot检测Mcl-1表达。【结果】相对于L-02细胞，Huh7、HePG2和PLC／PRF/5细胞IL-17AmRNA与蛋白水平明显增加，以HepG2细胞最高（P〈0．01）。rIL-17A使HepG2细胞凋亡减少（Pd0．01），MCl-1表达上调（P〈0．01）。MCl-1siRNA预处理则逆转rIL广17A对HepG2细胞凋亡的抑制作用（Pd0．05）。【结论】肝癌细胞IL-17A呈高表达，IL-17A通过上调MCl-1表达发挥抗HepG2细胞凋亡作用。[Objective]To explore the effects of interleukin-17A （IL-17A） on apoptosis of HepG2 cell and investigate the novel mechanism. [Methods]Quantitative real-time PCR （qRT-PCR） and Western blot were used to detect IL-17A expression in normal hepatocytes （L-02） and hepatic cancer cell lines （HuhT, HepG2 and PLC/PRF/5）. Subsequently, HepG2 ceils were selected and treated with recombinant IL-17A （rlL-17A） and/or myeloid cell leukemia-1 （Mcl-1） siRNA. Apoptotic rate was analyzed by flow cytometry. The expres- sion of Mcl-1 was measured by qRT-PCR and Western Blot. [Results] Compared to those in L-02 cells, the mRNA and protein levels of IL-17A were significantly increased in Huh7, HepG2 and PLC/PRF/5 cells, and HepG2 cells had the highest expression （ P 〈0.01）. Administration of rIL-17A reduced apoptotic rate and in- creased Mcl-1 expression in HepG2 cells （P 〈0.01）. However, siRNA-mediated knockdown of Mcl-1 re- versed the inhibitory effect of rlL-17A on HepG2 cell apoptosis （ P 〈0.05）.[Conclusion]IL-17A is highly ex- pressed in hepatic cancer cells. IL-17A reduces HepG2 cell apoptosis by upregulating Mcl-1 expression.

关 键 词：细胞凋亡/生理学 

分 类 号：R329.25[医药卫生—人体解剖和组织胚胎学]