加味附子理中汤抑制肺癌作用及基于网络基因/蛋白组学的抗肿瘤靶点预测  

Base on Mechanism and Modified Fuzi Lizhong Decoction for Inhibiting Lung Cancer Based on Network Gene/Proteomics

作　　者：李洪霖 黄莉[1] 马纯政[1] 吕默晗 李雁[2] LI Hong-lin;HUANG Li;MA Chun-zheng;LU Mo-han;LI Yan(Seconal Aftiliated Hospital of Henan University of Chinese Medicine,Zhenzhou,Henan 45000 China;Shanghai Municipal Hospital of Traditional Chinese Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 200071,China)

机构地区：[1]河南省中医院,河南郑州450002 [2]上海中医药大学附属市中医院,上海200071

出　　处：《时珍国医国药》2018年第9期2142-2146,共5页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金青年基金(81603590);河南省郑州市科技攻关项目(20150292);河南省中管局中医临床基地项目(2017JDZX016);上海市卫生局青年科研项目(20134Y187)

摘　　要：目的观察加味附子理中汤对肿瘤的抑制作用,并预测其作用靶点。方法加味附子理中汤及其拆方灌胃21天后对Lewis(LLC)小鼠皮下移植瘤的抑制作用,继而通过TCMDatabase、Bio Systems、Uniprot等数据库,建立加味附子理中汤作用的基因网络,利用Cytoscape 3.4及其插件进行网络可视化分析和数据挖掘,预测分析其分子和生物学功能。结果加味附子理中汤及其拆方人参荜茇组对LLC小鼠具有抑制作用,其作用机制可能与调控TGF-β1/SMAD2/3、Wnt/β/β-catenin、Gli/Hedgehog信号通路有关,作用的肿瘤主要有卵巢癌、肺癌、肝癌、恶性胶质瘤等。结论加味附子理中汤可以抑制Lewis(LLC)小鼠肿瘤具有抑制作用;并多目标调节肿瘤及肿瘤微环境分子网络以实现其特定的治疗效果。Objective We observed the inhibitory effect of flavored aconite on tumor and to predict its target.Methods The inhibitory effect of Fuzi Lizhong Decoction and its sub-recipes on Lewis（LLC） mice subcutaneously transplanted tumor after 21 days of gavage was followed by establishing a gene network of flavored Fuzi Lizhong Deciction via databases such as TCM Database,Bio Systems and Uniprot,Use Cytoscape 3.4 and its plug-ins for network visualization and data mining to predict and analyze molecular and biological functions.Results Modified Fuzi Lizhong Decoction and its disassembled Panax ginseng group had inhibitory effect on LLC mice,which may be related to the regulation of TGF-β1/SMAD2/3,Wnt/β/β-catenin,Gli/Hedgehog signaling pathway Related,the role of the tumor mainly ovarian cancer,lung cancer,liver cancer,glioblastoma and so on.Conclusion Fuzi Lizhong Decoction could inhibit Lewis（LLC） growth in mouse;and its multi-target regulate of tumor and the microenvironment molecular network achieving its specific therapeutic effect.

关 键 词：加味附子理中汤 基因/蛋白作用网络 Lewis(LLC)小鼠 信号通路 

分 类 号：R285.5[医药卫生—中药学]