针刺对支气管哮喘大鼠肺组织Fibronection、CollagenⅠ表达的影响  

Effect of acupuncture on expression of Fibronection and CollagenⅠin lung tissues of asthmatic rats

作　　者：张宇佳 李双 虞跃跃 韩君萍 赵叶 杨金华 崔建美 ZHANG Yu-jia;LI Shuang;YU Yue-yue;HAN Jun-ping;ZHAO Ye;YANG Jin-hua;CUI Jian-mei(Gaoping People＇s Hospital,Gaoping,048400,China;North China University of Science and Technology,Tangshan,063000,China)

机构地区：[1]高平市人民医院,山西高平048400 [2]华北理工大学中医学院,河北唐山063000

出　　处：《时珍国医国药》2018年第9期2291-2293,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(81303046);华北理工大学研究生创新项目(2017S48)

摘　　要：目的探讨针刺对支气管哮喘大鼠肺组织Fibronection、CollagenⅠ表达的影响。方法将30只SPF级雄性SD大鼠随机分为正常对照组、哮喘模型组和哮喘针刺组,每组10只。各组分别给予相应干预后,采用HE染色观察各组大鼠肺组织病理形态学改变,免疫组织化学染色法检测肺组织Fibronection、CollagenⅠ表达情况。结果针刺可以有效改善哮喘大鼠肺组织病理改变,哮喘针刺组大鼠肺组织Fibronection、CollagenⅠ的表达均较哮喘模型组降低(F=32.408,P=0.000; F=15.861,P=0.000)。结论针刺可降低哮喘大鼠肺组织中Fibronection、CollagenⅠ的过度表达以及改善哮喘大鼠肺组织的气道重塑程度。Objective To explore the effects of acupuncture on the expression of Fibronection and CollagenⅠ in lung tissues of asthmatic rats.Methods Thirty SPF male SD rats were randomly divided into normal control group,asthmatic model group and the acupuncture treatment group,with 10 rats in each group.Groups were given accordingly interventions,HE staining was applied to observe the morphologic changes of lung tissues;the immunohistochemical method was applied to test the protein expression of Fibronection and CollagenⅠ in lung tissue.Results Acupuncture can effectively improve pathological changes of lung tissues in asthmatic rats.The expression of Fibronection and CollagenⅠ in lung tissue of acupuncture group significantly lower than asthmatic group（F=32.408,P=0.000;F=15.861,P=0.000）.Conclusion Acupuncture could reduce the protein expression of Fibronection and CollagenⅠin lung tissues in asthmatic rats leading to airway remodeling.

关 键 词：针刺 支气管哮喘 纤维连接蛋白 Ⅰ型胶原蛋白 

分 类 号：R2-03[医药卫生—中医学]