糖胃安煎剂对糖尿病胃肠病变大鼠Cajal间质细胞和P物质的影响  

Effect of Tangweian Decoction on Cajal Interstitial Cells and Substance P in Diabetic Rats with Gastrointestinal Disease

作　　者：刘桂芳[1] 耿涛[1] 房玉涛[1] LIU Gui-fang;GENG Tao;FANG Yu-tao(Gang＇anmen Hospital,China Academy of Chinese Medical Sciences,Beijing 100053,China)

机构地区：[1]中国中医科学院广安门医院

出　　处：《中国实验方剂学杂志》2018年第22期127-132,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金青年基金项目(81302964/H2708)

摘　　要：目的：研究糖胃安煎剂对糖尿病胃肠病变大鼠胃排空的影响,并探讨其作用机制。方法：2~3月龄Wistar雄性大鼠50只,10只大鼠作为正常组,另外40只腹腔注射50 mg·kg-1链脲佐菌素（STZ）复制1型糖尿病动物模型,尾静脉随机血糖≥16.9 mmol·L-1者为糖尿病大鼠模型建立,随机分为糖尿病模型组,糖胃安煎剂高、中、低剂量（15,10,5 g·kg-1）组,每组10只,正常组和糖尿病模型组给予等量蒸馏水灌胃。8周后取材,采用放射性核素单光子发射断层显像技术（SPECT）测定胃排空,免疫组织化学技术观察胃肌间层Cajal间质细胞变化,蛋白质免疫印迹法（Western blot）检测下丘脑P物质蛋白表达。结果：与正常组比较,糖尿病模型组大鼠的胃排空显著降低,胃肌间层阳性Cajal间质细胞显著降低,下丘脑P物质蛋白表达降低（P〈0.01）。与糖尿病模型组比较,糖胃安煎剂高、中剂量组的胃排空率升高,Cajal间质细胞升高,下丘脑P物质蛋白表达均升高（P〈0.05,P〈0.01）;糖胃安煎剂低剂量组的胃排空率,Cajal间质细胞,下丘脑P物质蛋白表达有升高趋势,但无统计学意义。结论：糖尿病胃肠病变大鼠胃排空降低,胃肌间层Cajal间质细胞减少,下丘脑P物质表达降低,糖胃安煎剂可能通过增加大鼠胃肌间层Cajal间质细胞,提高下丘脑P物质水平,促进糖尿病胃肠病变大鼠胃排空,改善胃肠动力和功能。Objective： To study the effect of Tangweian decoction on gastric emptying in diabetic rats with gastrointestinal diseases,so as to explore its mechanism. Method： Fifty 2-3-month-old male Wistar rats were collected as subjects. Ten of them were randomly selected as normal control group（CON）,and the other 40 were made diabetic through intraperitoneal injection with streptozotocin（STZ,50 mg·kg）. Diabetes was verified after 1 week,and the animals with a blood sugar level higher than 16. 9 mmol·L-1 were considered as diabetic for the experiments. The 40 rats were randomly divided into diabetic control group（DM）,high-dose Tangweian decoction group（T1）,middle-dose group（T2）,and low-dose group（T3）（15,10,5 g·kg-1）. CON group and DM group were treated with distilled water by gavage. After 8 weeks,the gastric emptying rate was determined by single photon emission computed tomography（SPECT）. The immunohistochemistry was used to observe the changes of Cajal interstitial cells in the gastric myenteric layer. The hypothalamus substance P protein expression was detected by Western blot. Result： Compared with the CON group,the gastric emptying rate in DM group,T1 group,T2 group and T3 group decreased significantly（P〈0. 01）; the Cajal interstitial cells in gastric myenteric layer decreased significantly（P〈0. 05,P〈0. 01）; the hypothalamus protein expression substance P decreased significantly（P〈0. 05,P〈0. 01）. Compared with DM group,the gastric emptying rate and the Cajal interstitial cells in T1 and T2 groups were increased significantly（P〈0. 05,P〈0. 01）,while the expression of P substance in hypothalamus increased significantly（P〈0. 05,P〈0. 01）. For T3 group,the gastric emptying rate,Cajal interstitial cells and the expression of P substance in hypothalamus were increased,but with no statistically significant difference. Conclusion： Gastric emptying rate,Cajal interstitial cells in gastric myenteric layer,and the expression of substance P in hypothal

关 键 词：糖胃安煎剂 胃排空 CAJAL间质细胞 P物质 

分 类 号：R2-0[医药卫生—中医学] R22