基于肠-免疫-皮肤轴探讨洋金花对银屑病小鼠肠道Th17/Treg轴的影响  

The Effect of Datura metel L. on Intestinal Th17/Treg in Psoriasis Mice Based on Intestine-Immune-Skin Axis

作　　者：苏阳[1] 任文晨 李珊珊 吴伦[1] 高健 王秋红 程根宏[4] 陈晶[1] 匡海学[1] SU Yang;REN Wen-chen;LI Shan-shan;WU Lun;GAO Jian;WANG Qiu-hong;CHENG Gen-hong;CHEN Jing;KUANG Hai-xue(School of Pharmacy,Heilongjiang University of Chinese Medicine,Key Laboratory of Medicinal Materials,Chinese Academy of Sciences,Harbin 150040,China;Heilongjiang Medical Service Management Evaluation Center,Harbin 150040,China;School of Traditional Chinese Medicine,Guangdong Pharmaceutical University,Guangzhou 510000,China;University of California at Los Angeles,School of Microbiology and Immunology,Los Angeles 90095,CA)

机构地区：[1]黑龙江中医药大学药学院,黑龙江哈尔滨150040 [2]黑龙江省医疗服务管理评价中心,黑龙江哈尔滨150040 [3]广东药科大学中药学院,广东广州510000 [4]美国加州大学洛杉矶分校,美国加利福尼亚州洛杉矶90095

出　　处：《现代中药研究与实践》2018年第5期27-30,共4页Research and Practice on Chinese Medicines

基　　金：国家自然科学基金(81603416);中国博士后科学基金资助项目(2016M600267);第七批黑龙江省博士后特别资助(博士后青年英才计划:LBHTZ1619);黑龙江省普通本科高等学校青年创新人才项目(UNPYSCT-2016207);哈尔滨市科技创新人才专项资金项目(青年后备人才)项目(RC2017QN003037);黑龙江省博士后科研启动项目(LBH-Q17167)

摘　　要：目的探讨洋金花醉茄内酯类化合物对银屑病小鼠肠道中Th17/Treg细胞功能的影响。方法采用经典的银屑病小鼠模型,即咪喹莫特乳膏(IMQ)诱导小鼠背部皮肤产生银屑病样病理模型,并随机分为四组,即空白组、模型组、洋金花组和雷公藤阳性药物组。采用Psoriasis area and severity index(PASI)分析各组皮损严重程度;应用流式细胞法检测小鼠肠系膜淋巴结中Th17/Treg细胞数量表达;应用酶联免疫吸附法(ELISA法)依次检测肠粘膜中Th17/Treg细胞相关因子的含量。结果洋金花组能减少IMQ诱导的PASI评分,降低肠系膜中Th17细胞表达及相关细胞因子,而升高Treg细胞的表达及相关细胞因子。结论洋金花醉茄内酯类化合物可降低银屑病小鼠肠道Th17细胞功能,同时升高Treg细胞功能,进一步提示在肠-免疫-皮肤轴中,洋金花醉茄内酯类化合物通过影响肠道中的Th17/Treg细胞免疫平衡而达到防治银屑病的作用。Objective To investigate the effect of flower of Datura metel L. on the function of Th17/Treg cells in the intestine of mice psoriasis. Methods Psoriasis mice model are established using Imiquimod Cream, and randomly divided into four groups, namely control group, model group, Datura metel L. withanolides group and triptolide group. The psoriasis area and severity index（PASI） are analyzed with the severity of lesions; the expression quantity of Th17/Treg cells are detected by flow cytometry; enzyme-linked immunosorbent assay（ELISA） is used in the detection of Th17/Treg cells in the intestinal mucosa associated with the content. Results Group YWB reduced IMQ induced PASI score with lower Th17 expression and related cytokines, and meantime increased expression of Treg cells and relate cytokines. Conclusion Group YWB reduce intestinal Th17 cell function in mice psoriasis model, and increase the function of Treg cells in the meantime, further suggesting that YWB prevent psoriasis by affect Th17/Treg cells immune balance in the intestine-immune-skin axis.

关 键 词：银屑病 洋金花醉茄内酯类化合物 Th17/Treg细胞免疫平衡 肠-免疫-皮肤轴 

分 类 号：R285[医药卫生—中药学]