健骨颗粒乙酸乙酯萃取部位对卵巢切除大鼠骨质量的影响  

Effects of ethyl acetate extraction of Jiangu Granule in the bone quality of ovariectimized rats

作　　者：陈翔[1] 林燕萍[1] 贾晓康 王柄棋 孙雨晴[1] 黄云梅[1] 黄美雅[1] 魏振朴[1] CHEN Xiang;LIN Yan-ping;JIA Xiao-kang;WANG Bing-qi;SUN Yu-qing;HUANG Yun-mei;HUANG Mei-ya;WEI Zhen-pu(Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China)

机构地区：[1]福建中医药大学,福州350122

出　　处：《中华中医药杂志》2018年第11期4925-4928,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金资助项目(No.81574003);福建省科技厅引导性项目(No.2015Y0069)~~

摘　　要：目的:研究健骨颗粒乙酸乙酯萃取部位对卵巢切除大鼠骨质量的影响。方法:采用切除雌性SD大鼠双侧卵巢建立绝经后骨质疏松症模型。运用双能X线4500W骨密度仪测定骨密度,万能材料试验机检测骨组织的最大载荷。骨组织石蜡包埋切片后行Masson染色,观察骨组织形态,并做骨小梁面积百分比计量。通过酶联免疫吸附法检测血清中的骨碱性磷酸酶(BALP)、雌二醇(E2)、抗酒石酸酸性磷酸酶(TRAP)的含量。结果:与空白组比较,乙酸乙酯组术后4、12周BMD显著升高(P<0.01),术后12周胫骨三点抗弯最大载荷显著升高(P<0.01),术后8、12周腰椎、胫骨近干骺端骨小梁面积百分比及血清E2水平显著升高(P<0.01),术后12周血清BALP水平显著升高(P<0.01),术后8周血清TRAP水平显著下降(P<0.05)。结论:健骨颗粒乙酸乙酯萃取部位能有效的改善卵巢切除术后大鼠机体的骨代谢水平,改善骨质量。Objective： To observe the effects of ethyl acetate extraction of Jiangu Granule on the bone quality of ovariectimized rats. Methods： The postmenopausal osteoporosis rat model was established by removing bilateral ovaries of SD female rats. The bone density of bone tissue and biomechanics were detected. The morphology of the bone was observed by Masson staining. The concentration of E2, TRAP, and BALP in the serum was detected by the method of ELISA. Results： Compared with the blank group, the BMD at 4 and 12 weeks after surgery increased significantly（P0.01）, the maximum load of the tibia at 12 weeks after surgery increased significantly（P0.01）, the area percentage of bone trabeculae and E2 at 8 and 12 weeks after surgery increased significantly（P0.01）, the serum BALP level at 12 weeks after surgery increased significantly（P0.01）, the serum BALP level at 12 weeks after surgery increased significantly（P0.01）, the serum TRAP level at 8 weeks after surgery decreased significantly（P0.05）. Conclusion： The bone metabolism and bone quality of ovariectimized rats were effectively improved in ethyl acetate group.

关 键 词：健骨颗粒 萃取 卵巢切除大鼠 骨质量 骨质疏松症 绝经后骨质疏松症 

分 类 号：R285.5[医药卫生—中药学]