柴胡疏肝汤对脂多糖诱导的急性抑郁小鼠模型色氨酸代谢通路的影响  

Effects of Chaihu Shugan Decoction on tryptophan metabolic pathway in acute depressive mouse induced by lipopolysaccharide

作　　者：蔡伦[1] 胡玉英[1] 谭璐璐[1] 梁建平[1] 韦理萍[1] CAI Lun;HU Yu-ying;TAN Lu-lu;LIANG Jian-ping;WEI Li-ping(Department of Encephalopathy,The First Affiliated Hosipital of Guangxi University of Chinese Medicine,Nanning 530023,China)

机构地区：[1]广西中医药大学第一附属医院脑病科,南宁530023

出　　处：《中华中医药杂志》2018年第11期5212-5215,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：广西自然科学基金项目(No.2015GXNSFBA139172);广西壮族自治区中青年教师基础能力提升项目(No.2017KY0301)~~

摘　　要：目的:观察柴胡疏肝汤(CHSGD)对脂多糖(LPS)诱导的抑郁小鼠模型色氨酸(TRY)代谢通路的影响。方法:56只昆明小鼠随机分为对照组,模型组,米诺环素(MINO)组,1-甲基色氨酸(1-MT)组,CHSGD低、中、高剂量组(2.5、5、10g/kg),采用LPS造模。造模前72h,予药物治疗直至实验结束,观察小鼠造模后24h内的体质量变化,6h自由活动测试、强迫游泳试验(FST)和悬尾试验(TST)中抑郁行为学表现,检测脑组织白介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)和吲哚2,3双加氧酶(IDO)mRNA表达水平,五羟吲哚乙酸(5-HIAA)/5-羟色胺(5-HT)、5-HT/TRY及犬尿素(KYN)/TRY比值。结果:LPS诱导的抑郁小鼠模型出现24h内的体质量减轻,6h自由活动测试中活动计数减少,FST和TST中不动时间增加,脑组织IL-1β、TNF-α和IDO mRNA表达水平增加,5-HT/TRY比值下降,5-HIAA/5-HT和KYN/TRY比值升高(P<0.01)。CHSGD中、高剂量增加小鼠24h内的体质量和6h自由活动测试中活动计数,减少FST和TST中不动时间,抑制脑组织IL-1β、TNF-α和IDO mRNA表达,下调KYN/TRY比值,增加5-HT/TRY比值(P<0.05,P<0.01)。结论:CHSGD通过抑制脑IL-1β、TNF-α水平,进一步抑制IDO mRNA表达,正常化TRY代谢通路,改善LPS诱导的小鼠抑郁样行为。Objective： To investigate the effects of Chaihu Shugan Decoction（CHSGD） on tryptophan（TRY） metabolic pathway in acute depressive mouse induced by lipopolysaccharide（LPS）. Methods： Fifty-six mouse were divided into seven groups randomly, including control group, model group, minocycline group, 1-methyltryptophan（1-MT） group, CHSGD high, medium and low dose groups（2.5, 5, 10 g/kg）. Model mouse were treated by LPS. 72 h before LPS injection, mouse were received minocycline, 1-MT and CHSGD treatment until experiment finished. Weight changes in 24 h, performance in locomotor activity（LMA）, forced swim test（FST） and tail suspension test（TST） were recorded. Brain IL-1β, TNF-α and indoleamine 2,3-dioxygenase（IDO） mRNA expression, 5-hydroxyindoleacetic acid（5-HIAA）/5-hydroxytryptamine（5-HT）, 5-HT/TRY and kynurenine（KYN）/TRY ratios were caculated. Results： In model group, mouse weight in 24 h, activity counts in LMA were decreased, ＇immobility＇ time in FST and TST were increased; brain IL-1β, TNF-α and IDO mRNA expression were increased; 5-HT/TRY ratios were decreased and KYN/TRY ratio was upregulated（P0.01）. CHSGD（5, 10 g/kg） upregulated mouse weight and activity counts in locomotor activity, lowered ＇immobility＇ time in FST and TST, inhibited brain IL-1β, TNF-α and IDO mRNA expression, down-regulates the KYN/TR ratio, up-regulates 5-HT/TRY ratio（P0.01, P0.05）. Conclusion： CHSGD treats acute depression through down regulating brain IL-1β, TNF-α, IDO expression, normalizing TRY metabolic pathway and improving depressive behavior.

关 键 词：柴胡疏肝汤 抑郁 吲哚2 3双加氧酶 5-羟色胺 色氨酸 犬尿素 强迫游泳试验 悬尾试验 

分 类 号：R285.5[医药卫生—中药学] R-332[医药卫生—中医学]