鸽痘病毒的分离鉴定及病理组织学观察  

Pathogenic identification and pathological observations of pigeonpox virus in Fujian province

作　　者：池雪林[1] 陈珍珍 曾显成[1] 张翔 罗树红[2] 黄小红[1] CHI Xue-lin1 ,CHEN Zhen-zhen1 ,ZENG Xian-cheng1 ,Zhang Xiang1 ,LUO Shu-hong2 , HUANG Xiao- hong1(1. Key Laboratory for Integrated Chinese Traditional and Western Veterinary Medicine and Animal Healthcare in Fujian Province, College of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China;2. Department of Laboratory Medicine, School of Sto- matology and Medicine, Foshan University, Foshan, Guangdong 528000, China)

机构地区：[1]福建农林大学动物科学学院中西兽医结合与动物保健福建省高校重点实验室,福建福州350002 [2]佛山科学技术学院口腔医学院医学检验系,广东佛山528000

出　　处：《中国兽医学报》2018年第11期2078-2083,共6页Chinese Journal of Veterinary Science

基　　金：福建农林大学科技创新专项基金资助项目(KFA17221A,KFA17222A)

摘　　要：为确诊福建省某规模肉鸽养殖场疑似鸽痘疫情,采集病鸽皮肤痘痂组织,经处理后接种11日龄SPF鸡胚,培养7d后观察到鸡胚尿囊膜增厚,出现典型的白色痘斑。组织学观察可见皮肤表皮增生,形成厚的网状结构,上皮细胞呈不同程度的空泡变性,胞浆内有嗜酸性包涵体,呈红色、圆形或椭圆形;肺脏淤血,肺泡破裂及增殖性支气管炎。待检样本进行鸽痘病毒P4b基因PCR检测,得到特异性条带;根据P4b基因核酸序列构建系统发育树,结果显示分离株与来自美国（KC017965）和南非（KJ801920）的2株鸽痘病毒聚在一起,同源性为100%,与企鹅痘病毒、鹌鹑痘病毒和鸡痘病毒的同源性分别为99.6%,99.6%和91.1%;与金丝雀痘病毒和火鸡痘病毒同源性最低,为73.3%～75.0%。结果表明,本次暴发为鸽痘病毒感染所致,为鸽痘防控及致病机理研究奠定了理论基础。To diagnosis a suspected pigeonpox outbreak occurred in a scale of pigeon farm in Fujian province, pathogenic identification and histopathological observation were performed in this study. Thickening of allantois membrane and typical white acne marks were observed on 7 days after dif- ferent disposed. Samples of the pox-like skin scabs of sick pigeons were inoculated to 11 days old SPF chicken embryos. Histopathological features were observed including skin epidermal hyperpla- sia, thick reticular structure, varying degrees of vacuolar degeneration in the epithelial cells, and eo- sinophilic inclusion bodies in the cytoplasm,which were red,round or elliptical and gores,alveolar rupture and the proliferation bronchiolitis in the lung. Specific bands of P4b gene of pigeonpox vi- rus were observed. Phylogenetic analysis of P4b gene DNA sequences showed pigeonpox isolate in this study clustered with an American isolate （KC017965） and a South Africa isolate （KJ801920） together with 100% identity,and shared 99.6%,99.6% and 91.1% identity with the penguinpox virus,quailpox virus and chickenpox virus respectively,and shared low identities with canarypox virus and turkey pox virus （73.3%-75.0%）. The results confirmed that the cause of this outbreak was pigeonpox virus,which lays a theoretical foundation for the prevention and control of pigeon- pox and research on pathogenic mechanism.

关 键 词：鸽痘病毒 分离鉴定 病理观察 进化树分析 

分 类 号：S852.65[农业科学—基础兽医学]