初情期启动过程中小尾寒羊下丘脑KiSS-1基因甲基化状态与表达量相互关系  

Relationship between methylation status and expression of KiSS-1 gene in small tail han sheep during onset of puberty

作　　者：丁赫 宫永胜 王军[1] 吕文发[1] DING He,GONG Yong-sheng,WANG Jun ,LYU Wen-fa(Jilin Province Engineering Laborato-ry for Ruminant Reproductive Biotechnology and Healthy Production, College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China)

机构地区：[1]吉林农业大学动物科技学院吉林省反刍动物繁育生物技术与健康养殖工程实验室

出　　处：《中国兽医学报》2018年第11期2201-2204,共4页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(31402073);吉林省教育厅科学技术研究资助项目(JJKH20170319KJ)

摘　　要：KiSS-1基因在雌性动物初情期启动中扮演着重要作用,其启动子区甲基化状态与KiSS-1mRNA表达量之间关系尚不清楚。本试验利用亚硫酸氢盐测序（bisulfite sequencing PCR,BSP）技术和荧光定量PCR技术研究了小尾寒羊初情期前、临近初情期和初情期母羊下丘脑KiSS-1基因启动子区甲基化状态和KiSS-1基因mRNA表达量,分析两者之间的关系。结果表明：随着初情期的启动,KiSS-1基因启动子区甲基化水平显著降低,尤其是在启动子区-501位点降低最明显,并且KiSS-1基因mRNA表达量随着初情期的启动显著升高。结果提示：初情期的启动可能通过降低下丘脑KiSS-1基因启动子区特定位点甲基化水平,从而提高KiSS-1基因mRNA表达量。The KISS-1 ever, the relationship gene plays an important role in the onset of puberty in female animals. How- between the methylation status of KISS-1 promoter and the expression of KISS-1 mRNA during puberty onset is unclear. In this study, the methylation status of KISS-1 gene promoter and the expression of KISS-1 mRNA in hypothalamus of ewes in different stages （peri-puberty,pre-puberty and puberty）of small tail han sheep was research by using bisulfite se- quencing PCR（BSP）and reahime fluorescence quantitative PCR. The results showed that with the onset of puberty, the methylation level of KISS-1 gene promoter region significantly decreased, es- pecially in the promoter region-501, while the expression of KISS-1 mRNA increased significantly. The studies indicated that the increasing expression of KISS-1 mRNA may be cuased by decreasing the methylation level of the specific site in the promoter region of KISS-1 gene in hypothalamus during the onset of puberty in sheep.

关 键 词：初情期 下丘脑 KISS-1 甲基化 羊 

分 类 号：S814.8[农业科学—畜牧学]