3D打印复合材料修复兔大段胫骨缺损组织学、影像学和生物力学研究  

Experimental study on histological, imaging and biomechanical properties of 3D printed composite material for repairing large segmental tibial defects in rabbits

作　　者：耿洋 张春霖[2] 高山 席俊伟 Geng Yang;Zhang Chunlin;Gao Shan;Xi Junwei(The First Department of Orthopedics,Center Hospital of Zhumadian,Zhumadian 463000,Chin;The Fifth Department of Orthopedics,the First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China)

机构地区：[1]河南省驻马店市中心医院骨一科,463000 [2]郑州大学第一附属医院骨五科,450052

出　　处：《中华实验外科杂志》2018年第11期1999-2002,共4页Chinese Journal of Experimental Surgery

摘　　要：目的探讨3D打印羟基磷灰石和聚己内酯复合材料修复兔大段胫骨缺损的效果。方法将18只兔随机平均分为空白组、对照组和实验组。复制兔大段胫骨缺损模型。以羟基磷灰石和聚己内酯为复合材料3D打印仿生胫骨。对照组用兔自身骨进行修复，实验组用3D打印的仿生胫骨修复。术后6周和12周对胫骨进行X线影像学检查，术后12周对进行进行组织学和生物力学检查。结果术后对照组和实验组伤口生长良好，无感染；术后对照组和实验组没有见到显著成角和骨位移，有明显的新骨生成，术后12周对照组和实验组的骨缺损结合处骨端完全融合；术后实验组和对照组组织切片内均有新骨组织形成，有骨窝细胞存在，骨小梁相互之间连接成网络，并且有矿化表现，在新生的骨组织内部和周围有微小的血管生成；术后12周兔缺损部位的胫骨最大载荷空白组为（581．35±58．41）N、对照组为（577．52±55．96）N，实验组为（570．86±60．43）N，组间差异无统计学意义（P=0．952）；兔缺损部位的胫骨最大载荷时吸收的能量空白组为（0．83±0．18）J、对照组为（0．81±0．17）J，实验组为（0．80±0．19）J，组间差异无统计学意义（P=0．958）。结论采用3D打印聚己内酯和羟基磷灰石复合材料制备仿生胫骨来修复打断胫骨缺损时，可以获得与白体骨移植修复相似的组织学、影像学和生物力学效果。Objective To explore the effect of 3D printing hydroxyapatite and polycaprolaetone composite material on repairing large tibial defects in rabbits. Methods Eighteen rabbits were randomly divided into blank group, control group and experimental group. The model of large segmental tibial defect in rabbits was established. Hydroxyapatite and polycaprolaetone were used as composite materials to print 3D biomimetic tibia. The control group was repaired with rabbit＇ s own bone, and the experimental group was restored with 3D printed bionic tibia. X-ray examination of tibia was performed at 6 and 12 weeks after operation. Histological and biomechanical examinations were performed 12 weeks after operation. Results After operation, the wounds in the control group and the experimental group grew well without infection. There was no significant angle and bone displacement in the control group and the experimental group, and there was a significant new bone formation. At 12th week after the operation, the bone ends of the bone defect in the control group and the experimental group were completely fused. New bone tissue was formed in the tissue sections of the experimental group and the control group. The ceils of the bone fossa existed, the trabeculae were connected each other into a network, and there was mineralization, and there was small angiogenesis in and around the new bone tissue. There was no significant difference between the blank group [ （581.35±58.41 ） N], the control group [ （577. 52±55.96） N] and the experimental group [ （570. 86±60. 43） N] for tibial maximum load at 12th week after operation in rabbits （P = 0. 952）. There was no significant difference between blank group [ （0. 83±0. 18） J], the control group [ （0. 81±0. 17） J] and experiment group [ （0. 80±0. 19） J] for absorbed energy of the tibial maximum load at 12th week after operation in rabbits （P =0. 958）. Conclusion The bionic tibia is prepared by 3D printing of polycaprolactone and hydroxyapatite composite to

关 键 词：胫骨缺损 3D打印 兔 组织学 影像学 生物力学 

分 类 号：R687.3[医药卫生—骨科学]