间充质干细胞干性维持的研究进展  

Advances in research on the maintenance of the sternness of mesenchymal stem cells

作　　者：郑力铭 宋凯[1,2] 蒋青 Zheng Liming;Song Kai;Jiang Qing(Department of Sports Medicine and Adult Reconstructive Surgery,Drum Tower Hospital,School of Medicine,Nanjing University,Nanjing 210008,China;Laboratory for Bone and Joint Disease,Model Animal Research Center（MARC）,Nanfing University,Nanjing 210093,China)

机构地区：[1]南京大学医学院附属鼓楼医院运动医学与成人重建外科,210008 [2]南京大学模式动物研究所骨关节疾病实验室,210093

出　　处：《中华实验外科杂志》2018年第11期2165-2169,共5页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金（81420108021、81730067、81622033、81702151）;江苏省自然科学基金（BK20170121）;中央高校基本科研业务费专项资金（021414380306）

摘　　要：随着组织工程学的发展，间充质干细胞作为最常用的种子细胞，在组织损伤后修复中有着较好的应用前景。但在干细胞体外培养扩增及移植人体内参与组织修复的过程中，干细胞自身的干性下降，难以较好分化成为特定的组织类型始终是一个难题。因此本文对间充质干细胞干性的影响因素、评估方法以及干性维持的机制和方法进行综述。Mesenchymal stem cells, acting as one of the most common cells used in tissue engineering, can be easily obtained and cultured. However, when cultured in vitro,sternness of mesenchymal stem cells decreased rapidly, which limits its application in repair of injuries. Therefore, finding the factors adjusting stemness of mesenchymal stem cells and maintaining its capability of differentiating into particular tissues are critical in tissue engineering. This article summarized related factors and methods, which may be employed to evaluate and maintain the stemness of mesenchymal stem cells.

关 键 词：间质干细胞 组织工程 细胞分化 细胞衰老 多能干细胞 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]