基于液相色谱-质谱联用的血清代谢组学在中医上火研究中的应用  

Serum Metabolomics Study of "Shanghuo" in Traditional Chinese Medicine Based on the Liquid Chromatography-Mass Spectrometry

作　　者：吴德鸿 周佳[2] 李倩倩[2] 谭祖教 范永升[2] WU Dehong;ZHOU Jia;LI Qianqian(The Second Affiliated Hospital of Zhejiang Chinese Medical University,Hangzhou（310005,China;School of Basic Medical Sciences,Zhejiang Chinese Medical University)

机构地区：[1]浙江中医药大学附属第二医院,杭州310005 [2]浙江中医药大学基础医学院

出　　处：《浙江中医药大学学报》2018年第10期769-774,共6页Journal of Zhejiang Chinese Medical University

基　　金：国家重点基础研究发展计划(973计划)项目(2014CB543001);国家自然科学基金项目(81703966);浙江省中医药科技计划项目(2017ZA064)~~

摘　　要：[目的]利用代谢组学表征上火人群血清代谢组的特异性变化,为进一步探索上火的发病机制提供参考依据。[方法]采集30例上火和24例非上火人群血清样本,采用液相色谱-质谱联用(liquid chromatography-mass spectrometry,LC-MS)技术对血清中代谢物进行检测,并结合正交信号校正-偏最小二乘法判别分析(orthogonal signal correction-partial least squares discriminant analysis,OSC-PLS DA)和t检验挖掘上火后发生显著变化的血清代谢物,进一步进行结构鉴定,解释上火状态下的人体代谢变化的病理生理机制。[结果]结合单变量和多变量统计分析手段筛选出上火与非上火人群中存在显著差异的代谢物,部分代谢物结构已经确认,主要是脂类物质,包括2种磷脂酰胆碱(34:1和38:7)、2种溶血磷脂酰胆碱(18:1和16:0)、1种磷脂酰乙醇胺(34:2)、1种溶血磷脂酰乙醇胺(18:2)、2种鞘脂(44:2和40:2)、1种甘油三酯(53:3)及胆红素等。[结论]利用LC-MS技术从整体水平上探讨上火人群血清代谢谱的变化,发现多种脂质和胆红素有一定程度地下降,可能与上火人群体内炎性反应和氧化应激的发生有关。[Objective] To characterizing the metabolic profile under ＂Shanghuo＂（SH） state condition with metabolomics technology, and elucidate the mechanism of SH. [Methods] In the study, liquid chromatography-mass spectrometry（LC-MS） technology was employed to collected the metabolic data.Serums from 30 subjects with TCM heat syndrome（SH group） and 24 healthy controls（control group） were analyzed. Orthogonal signal correction-partial least squares discriminant analysis（OSC-PLS DA） and student＇s t-test were combined to screen the significant differences between two groups. Moreover,the structures of these metabolites were identified to explain the response of human body under the state of SH. [Results] There were significantly different metabolic profiles between SH patients and controls, and part of the metabolites have been structurally identified, mainly were lipids, such as phosphatidylcholines（34：1 and 38：7）, lysophosphatidylcholines（18：1 and 16：0）, phosphatidylethanolamine（34：2） lysophosphatidylethanolamine（18：2）,sphingolipids（44：2 and 40：2）, triglyceride（53：3） and bilirubin. [Conclusion] Differences of serum metabolites have been found in the serum from SH patients, and the decreased levels of some lipids and bilirubin may be caused by the inflammatory reaction and oxidative stress.

关 键 词：上火 血清 LC-MS技术 代谢组学 脂质 正交信号校正-偏最小二乘法判别分析 

分 类 号：R720[医药卫生—儿科]