红参、黄芪诱导的大鼠实热证模型血液内环境改变及分析  

Analysis of the Changes of Serum Metabolites in Model Rats with Heat Syndrome Induced by Radix Ginseng Rubra and Radix Astragali

作　　者：陈仙英 徐莉[1] 李思敏[2] 汪琴静 包洁[1] 季巾君[1] 谢冠群[1] 范永升[1] CHEN Xianying;XU Li;LI Simin(Zhejiang Chinese Medical University,Hangzhou（310053）China;The Third Hospital Affiliated to Zhejiang Chinese Medical University;Jiangnan Hospital Affiliated to Zhejiang Chinese Medical University)

机构地区：[1]浙江中医药大学,杭州310053 [2]浙江中医药大学附属第三医院 [3]浙江中医药大学附属江南医院

出　　处：《浙江中医药大学学报》2018年第10期790-796,共7页Journal of Zhejiang Chinese Medical University

基　　金：国家重点基础研究发展计划(973计划)项目(2014CB543001;02);国家自然科学基金项目(81673857)~~

摘　　要：[目的]检测红参、黄芪诱导的大鼠实热证模型血液内环境氧化应激状态的变化及其血液内小分子代谢物的改变,探讨实热证的病理生理机制。[方法]将清洁级雄性Wistar大鼠40只随机分为实热组和正常对照组两组,每组各20只。实热组采用红参、黄芪水煎剂灌胃构建实热证大鼠模型,正常对照组予等量的0.9%氯化钠溶液灌胃,两组灌胃均为2次/d,共1周。测量大鼠日均饮水量、肛温;ELISA检测血清中促甲状腺激素(thyroid stimulating hormone,TSH)及17-羟皮质类固醇(17-hydroxycorticosteroid,17-OHCS)含量;硫代巴比妥法检测血浆中氧化应激指标丙二醛(malondialdehyde,MDA)的水平;化学比色法检测血浆总抗氧化能力(total antioxidant capacity,T-AOC);分光光度计检测血浆中谷胱甘肽(glutathione,GSH)的变化;液相色谱-质谱联用(liquid chromatography-mass spectrometry,LC-MS)鉴定血清中小分子代谢物的改变。[结果]实热组大鼠在灌胃期间饮水量增多(P<0.05),血清内TSH及17-OHCS含量均高于正常对照组(P<0.01),氧化应激指标MDA、GSH、T-AOC水平明显高于正常对照组(P<0.05)。LC-MS分析显示,与正常对照组比较,实热组大鼠血清中溶血卵磷脂(lysophosphalipid,Lyso PC)、二十碳五烯酸(eicosapentaenoic acid,EPA)、皮甾酮等物质含量均降低(P<0.01);棕榈酸、鞘氨醇含量升高(P<0.05,P<0.01)。[结论]红参、黄芪可引起实热证大鼠的氧化应激水平升高,脂质代谢紊乱,导致血液中不饱和脂肪酸降低,这些改变有加剧血管损伤的风险。[Objective] To detect the level of oxidative stress state and the changes of serum metabolites in model rats with heat syndrome induced by Radix Ginseng Rubra and Radix Astragali, and to explore the pathophysiological mechanism of heat syndrome.[Methods] Forty male Wistar rats were randomly divided into two groups： real heat group and control group, with 20 rats in each group. In real heat group, a rat model of heat syndrome was established by intragastric administration of Radix Ginseng Rubra and Radix Astragali decoction. The rats in control group were given an equal amount of 0.9% sodium chloride solution and administered twice a day for 1 week. The daily average drinking water and anal temperature of the rats were measured. Serum thyroid stimulating hormone（ TSH） and 17-hydroxycorticosteroid（ 17-OHCS） were detected by ELISA. Determination of oxidative stress index malondialdehyde（MDA） in plasma by thiobarbital assay; chemical colorimetry to detect total antioxidant capacity（T-AOC）; spectrophotometer to detect changes in plasma L-glutathione（GSH）; liquid chromatography-mass spectrometry（LC-MS） determination of changes in small molecule metabolites in serum. [Results] In real heat group, the amount of drinking water increased during gavage, and the contents of TSH and 17-OHCS in serum were higher than those in control group（P0.05,P0.01）. Compared with the rats in control group, the levels of MDA, GSH and T-AOC were significantly increased in serum of real heat group（P〈0.05）. LC-MS analysis of differential metabolites showed that compared with control group, the serum levels of lysophosphalipid（Lyso PC）, eicosapentaenoic acid（EPA） and cortexolone were decreased in the rats of real heat group（ P〈0.01）. Palmitic acid and sphingosine increased（P0.05,P0.01）. [Conclusion] Radix Ginseng Rubra and Radix Astragali caused the oxidative stress level and the lipid metabolism disorder in rats with heat syndrome, which led to the decrease of unsaturated fatty acids in the

关 键 词：红参 黄芪 实热证 上火 氧化应激 脂质代谢 不饱和脂肪酸 血液内环境 

分 类 号：R720[医药卫生—儿科]