体外制备原始生殖细胞来源类胚体的一种方法  

In vitro preparation of embryoid body from primordial germ cell

作　　者：扈清云[1] 王培军[1] 朱金玲[1] 陈乃峰[1] 韩曦[1] 钟堂武[1] 张东东[1] HU Qing-yun;WANG Pei-jun;ZHU Jin-ling;CHEN Nai-feng;HUAN Xi;ZHONG Tang-wu;ZHANG Dong-dong(Preclinical Medical of Jiamusi Univerity,Jiamusi 154007 China)

机构地区：[1]佳木斯大学基础医学院,黑龙江佳木斯154007

出　　处：《解剖学研究》2018年第5期380-384,F0004,共6页Anatomy Research

基　　金：佳木斯大学科学技术研究项目(13z1201532);佳木斯大学创新团队(Cxtdpy-2016-02)

摘　　要：目的探讨一种利用鸡原始生殖细胞作为种子细胞,体外制备类胚体的方法。方法采用干细胞体外培养获得干细胞系并制备类胚体,采用免疫荧光染色、HE染色、RT-PCR及诱导分化等技术对干细胞系及类胚体的生物学特性进行鉴定分析。结果通过悬浮培养获得了最大体积为440μm的类胚体,并借助共聚焦显微镜活体细胞成像系统捕捉到其向心肌分化后自主收缩的影像。结论本研究提供了一种快速、高效的体外制备大体积类胚体的技术方法。Objective To prepare a novel method that preparing embryoid bodies by primordial germ cells in vitro.Methods The stem cell culture and inducing technologies were performed,the immunofluorescent staining,HE staining,RT-PCR and confocal laser scanning were utilized to identify the biological characteristics of primordial germ cells and embryoid bodies. Results Embryoid bodies with big size（maximum：440 μm in diameter）were obtained through the protocol.Conclusion This study provide a new technical platform to prepare embryoid bodies with big size in a short period with high efficiency.

关 键 词：干细胞 原始生殖细胞 类胚体 

分 类 号：R329.1[医药卫生—人体解剖和组织胚胎学]