高脂小鼠脂肪肝组织油红O特殊染色的应用  

Oil-red O special staining techniques applied in fatty liver tissue of hyperlipidemic mouse

作　　者：钟晓怡 林云 郭艳 蒲蕊 曹广文 殷建华 ZHONG Xiao-yi;LIN Yun;GUO Yan;PU Rui;CAO Guang-wen;YIN Jian-hua(Department of Epidemiology,The Second Military Medical University,Shanghai 200433,China)

机构地区：[1]海军军医大学流行病学教研室,上海200433

出　　处：《上海预防医学》2018年第10期873-877,共5页Shanghai Journal of Preventive Medicine

基　　金：国家重点基础研究发展计划(2015CB554005);国家自然科学基金项目(81373067)

摘　　要：【目的】探讨2种油红O染色法在非酒精性脂肪肝病中脂肪变性检测的应用。【方法】选取非酒精性脂肪肝病动物疾病模型C57BL/6J小鼠30只,安乐死处理后摘取左肝叶,将肝组织用4%多聚甲醛固定液充分固定后分为2份进行组织学制片,1份样品制作石蜡切片,石蜡切片制备完成后进行苏木精-伊红(HE)染色,作为脂肪变性的鉴定,另1份样品用30%蔗糖溶液脱水后用OCT包埋制作冰冻切片,冰冻切片制备完成后进行2种方法的油红O染色。通过盲测的方法,在光学显微镜下观察油红O在脂滴细胞的定位情况,并对2种染色方法进行对比。【结果】2种染色方法对组织及细胞没有任何病理性的损伤,并具有较好的显色与定位作用。【结论】运用良好的特殊染色技术,可以清晰分辨脂滴细胞,并显示出脂滴细胞的数量,从而对非酒精性脂肪肝病中的脂肪变性进行定位与半定量的分析。[ Objective ] To explore the application of two oil-red staining methods for steatosis in non-alco- holic fatty liver disease. [ Methods] Thirty C57BL/6J mice models of non-alcoholic fatty disease were selected. Alter euthanasia, the left liver lobe was harvested immediately, which was immersed in a large volume of 4% paraformaldehyde fixative in eaeodylate buffer. Alter fixation, the liver was divided into two samples for making histological sections. One sample was made into paraffin sections for hematoxyin-eosin （HE） staining to identify steatosis. The other was made into frozen sections of OCT-embedded block alter dehydration with 30% sucrose solution. The frozen sections were stained by two oil-red O staining methods. The localization of oil-red O in the lipid droplet was observed by blind measurement under microscope, and the results obtained by the two staining methods were compared. [ Results ] The two staining methods proved to be without pathological damage to tissue and cell, and had good coloration and localization effect, which provided scientific basis for the diagnosis of non-alcoholic fatty liver disease. [ Conclusion ] By the use of good special staining technology, the lipid drop cells are distinguished clearly, and the number of lipid drop cells are displayed. The steatosis in non-alcoholic tarry disease is located and analyzed with half quantification.

关 键 词：脂肪变性 油红O染色 特殊染色 非酒精性脂肪肝病 

分 类 号：R446[医药卫生—诊断学]