血清单核细胞趋化蛋白-1、肿瘤坏死因子-α和转化生长因子-β1的检测在早期糖尿病肾病中的诊断价值  

The diagnostic value of serum monocyte chemoattractant protein-1,tumor necrosis factor-α and transforming growth factor-β1 in early diabetic nephropathy

作　　者：刘金花[1] 孟祥云[2] 吴丽丽[3] LIU Jinhua;MENG Xiangyun;WU Lili(Jiuquan People＇s Hospital,Jiuquan,Gansu 735000,China;Gansu Hospital of Traditional Chinese Medicine,Lanzhou,Gansu 730050,China;Beijing University of Chinese Medicine,Beijing 100029,China)

机构地区：[1]甘肃省酒泉市人民医院,甘肃酒泉735000 [2]甘肃省中医院,甘肃兰州730050 [3]北京中医药大学,北京100029

出　　处：《国际检验医学杂志》2018年第22期2756-2758,共3页International Journal of Laboratory Medicine

基　　金：国家自然科学基金资助项目(81560764);北京市教育委员会共建项目(2013039)

摘　　要：目的探讨2型糖尿病患者血清单核细胞趋化蛋白-1(MCP-1)、肿瘤坏死因子-α(TNF-α)和转化生长因子-β1(TGF-β1)的检测在早期糖尿病肾病诊断中的临床价值。方法选择2型糖尿病患者120例,根据尿白蛋白排泄率(UAER)将其分为UAER正常组、UAER增高组,另选健康体检者60例作为对照组,ELISA法检测3组研究对象血清MCP-1、TNF-α与TGF-β1的表达水平,并分析各指标的阳性率,比较单独检测与联合检测在诊断糖尿病肾病中的效率。结果 UAER正常组MCP-1、TNF-α与TGF-β1表达水平显著高于对照组,差异有统计学意义(P<0.05)。UAER增高组患者的MCP-1、TNF-α与TGF-β1水平显著高于UAER正常组,差异有统计学意义(P<0.05)。UAER增高组患者的MCP-1、TNF-α与TGF-β1的阳性率均显著高于其他两组,差异有统计学意义(P<0.05)。3项指标联合检测对糖尿病肾病的灵敏度及特异度均显著高于单一检测结果,差异有统计学意义(P<0.05)。结论 MCP-1、TNF-α与TGF-β1的检测对早期糖尿病肾病的诊断具有一定参考价值,3项指标联合检测的诊断价值高于单一检测。Objective To investigate clinical value of the expressions of monocyte chemoattractant protein 1 （MCP 1） ,tumor necrosis factor -α （TNF-α） and transforming growth factor- β1 （TGF-β1） in patients with type2 diabetes mellitus on the diagnosis of nephropathy. Methods 120 patients with type2 diabetesmel litus were selected and divided into two groups according to the urinary albumin excretion rate （UAER） ：nor real UAER group, and UAER high group. 60 healthy subjects were selected as the control group. Serum MCP 1, TNF -α and TGF -β1 expression levels were tested by ELISA assay. The positive rate of each index was analyzed, and the efficiency of single detection and combined detection in the diagnosis of diabetic nephropathy was compared. Results The levels of MCP 1,TNF -α and TGF -β1 in normal UAER group were significantly higher than those in control group, and the difference was statistically significant （P〈0.05）. The levels of MCP 1,TNF-α and TGF -β1 in patients with elevated UAER were significantly higher than those in patientsnephropathy. The diagnostic value of combined detection ..

关 键 词：单核细胞趋化蛋白-1 肿瘤坏死因子-Α 转化生长因子-Β1 糖尿病肾病 诊断 

分 类 号：R587.2[医药卫生—内分泌]