隔山消水溶性部位对阿托品抑制小鼠胃肠功能的影响  

The Inhibitory Effects of Water-soluble Fraction of Cynanchum Wilfordii on Atropine-induced Gastrointestinal Disfunction in Mice

作　　者：刘亭[1,2] 杨淑婷 黎娜 潘洁[2,4] 陆苑[1,2] 李勇军[2,4] 钟民宽 孙佳[1,2] LIU Ting;YANG Shuting;LI Na;PAN Jie;LU Yuan;LI Yongjun;ZHONG Minkuan;SUN Jia(Guizhou Provincial Key Laboratory of Pharmaceutics,Guizhou Medical University,Guiyang 550004,Guizhou,China;State Key Laboratory of Functions and Applications of Medicinal Plants,Guizhou Medical University,Guiyang 550004,Guizhou,China;School of Pharmacy,Guizhou Medical University,Guiyang 550004,Guizhou,China;Engineering Research Center for the Development and Application of Ethnic Medicine and TCM（Ministry of Education）,Guizhou Medical University,Guizhou Medical University,Guiyang 550004,Guizhou,China)

机构地区：[1]贵州医科大学贵州省药物制剂重点实验室,贵州贵阳550004 [2]贵州医科大学药用植物功效与利用国家重点实验室,贵州贵阳550004 [3]贵州医科大学药学院,贵州贵阳550004 [4]贵州医科大学民族药与中药开发应用教育部工程研究中心,贵州贵阳550004

出　　处：《贵州医科大学学报》2018年第11期1252-1255,共4页Journal of Guizhou Medical University

基　　金：国家自然科学基金项目(81760700/81760699);黔科合平台人才[(2016)5613\5677]

摘　　要：目的:研究隔山消水溶性部位对阿托品所致小鼠胃肠运动及胃肠激素分泌抑制的影响。方法:将实验小鼠随机分为空白组、模型组(硫酸阿托品)、阳性药组(多潘立酮组)、隔山消水溶性部位低、中、高剂量组,多潘立酮组以0. 5 mg/10 g灌胃给予多潘立酮混悬液,隔山消低、中、高组灌胃分别给予0. 18、0. 36及0. 72 g/kg隔山消水溶性部位提取物混悬液,空白组和模型组均给予生理盐水,连续给药4 d,每天2次;末次给药30 min后,除空白组外其余5组小鼠均腹腔注射硫酸阿托品建立胃肠功能障碍模型(0. 5 mg/kg),取血、取胃及小肠组织,采用酚红测定各组胃排空率、小肠推进率,同时检测血清中胃动素(MTL)、胃泌素(GAS)、血管活性肠肽(VIP)的含量。结果:与模型组相比,隔山消水溶性部位低、中、高剂量组胃排空率和小肠推进率均显著提高(P<0. 01),其中中剂量组与多潘立酮组相当;胃肠激素水平测定结果表明隔山消水溶性部位中、高剂量组显著提高了模型小鼠的MTL、GAS含量(P <0. 05或P <0. 01),降低了VIP含量(P <0. 05)。结论:隔山消水溶性部位提取物能促进小鼠胃排空和肠推进,其机制可能与促进胃肠道MTL及GAS的释放和抑制VIP的释放有关。Objective：To study the effects of water-soluble fraction of Cynanchum wilfordii on atropine-induced gastrointestinal motility and gastrointestinal hormones in mice. Methods： The mice were randomly divided into the blank group, the model group, the domperidone group and three groups of water-soluble fraction of Cynanchum wilfordii with low dose, medium dose and high dose （0.18, 0.36 and 0.72 g/kg） . In the inhibiting state caused by atropine, the content of phenol red was determined to measure the rate of gastric emptying and small intestinal propulsion and serum levels of the motilin （MTL）, gastrin （GAS） and vasoactive intestinal peptide （VIP） were measured in mice. Results： Compared with the blank group, the rates of gastric emptying and small intestinal propulsion significantly increased in the low, medium and high dose groups （ P 〈0.01）, and the medium dose group was equivalent to the positive one. The MTL and GAS significantly increased （ P 〈0.05 or P 〈0.01） and VIP decreased significantly （ P 〈0.05） in the medium and high dose groups. Conclusions： Water-soluble fraction of Cynanchum wilfordii can promote the gastric emptying and small intestinal propulsion, and its mechanism is likely to be related with the promotion of MTL and GAS levels and the release of VIP level in gastrointestinal tract.

关 键 词：隔山消 水溶性部位 胃排空 肠推进 胃动素 胃泌素 血管活性肠肽 

分 类 号：R29[医药卫生—民族医学] R244[医药卫生—中医学]