口蹄疫灭活病毒配合枯草芽孢杆菌对提高牛呼吸道先天免疫力的研究  

Intranasal immunization with inactivated foot-and-mouth disease virus and Bacillus subtilis improve innate immunity of cattle respiratory tract mucosa

作　　者：李乙江 张泉鹏 苗海生 段体祥 张应国 杨柱昌 李世钰 冯德正 陈德寿 雷锦文 高花英 鲍晓伟 李作生 杨倩[2] LI Yi-jiang;ZHANG Quan-peng;MIAO Hai-sheng;DUAN Ti-xiang;ZHANG Ying-guo;YANG Zhu-chang;LI Shi-yu;FENG De-zheng;CHEN De-shou;LEI Jin-wen;GAO Hua-ying;BAO Xiao-wei;LI Zuo-sheng;YANG Qian(Dehong Animal Disease Control Center,Dehong 678400,China;Animal Medicine College,Nanjing Agricultural University,Nanjing 210095,China;Kunming Animal Health Inspection,Kunming 650000,China;Yunnan Animal Sience and Veterinary Institute,Kunming 650000,China;Agricultural Comprehensive Service Center of Fengpin Town,Dehong 678400,China;Agricultural Comprehensive Service Center of Jinghan Town,Dehong 678700,China;Mangshi County Animal Disease Control Center,Dehong 678400,China;School of Agriculture Yunnan University,Yunnan University,Kunming 650500,China;Longchuan County Animal Disease Control Center,Dehong 678700,China;Agricultural Comprehensive Service Center of Xinchen Town,Dehong 679300,China;Agricultural Comprehensive Service Center of Zanxi Town,Dehong 679300,China;Wuhan Chopper Biology CO.,LTD,Wuhan 430000,China)

机构地区：[1]德宏州动物疫病预防控制中心,云南德宏678400 [2]南京农业大学动物医学院,江苏南京210095 [3]昆明市动物卫生监督所,云南昆明650000 [4]云南省畜牧兽医科学院,云南昆明650000 [5]风平镇农业综合服务中心,云南德宏678400 [6]景罕镇镇农业综合服务中心,云南德宏678700 [7]芒市动物疫病预防控制中心,云南德宏678400 [8]云南大学农学院,云南昆明650500 [9]陇川县动物疫病预防控制中心,云南德宏678700 [10]新城乡农业综合服务中心,云南德宏679300 [11]盏西镇农业综合服务中心,云南德宏679300 [12]武汉中博生物股份有限公司,湖北武汉430000

出　　处：《中国预防兽医学报》2018年第10期934-939,共6页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家自然科学基金项目(31372465)

摘　　要：本研究采用O型口蹄疫灭活病毒配合枯草芽孢杆菌喷鼻气雾免疫健康牛,并设单独使用枯草芽孢杆菌组、灭活病毒、常规疫苗免疫组和空白对照组,评价O型口蹄疫灭活病毒配合免疫增强剂枯草芽孢杆菌通过喷鼻气雾免疫健康牛对其呼吸道先天免疫力的影响。通过免疫组织化学染色显示,免疫后灭活病毒配合免疫增强剂试验组牛鼻黏膜与肺内支气管黏膜中TLR7的表达和CD3^+淋巴细胞呈极显著增多(p<0.01);而常规口蹄疫灭活苗对其TLR7的表达和CD3^+淋巴细胞数量的影响不显著(p>0.05)。ELISA检测结果显示,与对照组相比,灭活病毒配合免疫增强剂试验组牛呼吸道组织中IL-12水平呈极显著增加(p<0.01),IL-4和IL-10水平无明显差异(p>0.05);而常规口蹄疫灭活苗对牛呼吸道组织中IL-4、IL-10和IL-12的影响不明显(p>0.05)。表明O型口蹄疫灭活病毒配合枯草芽孢杆菌能够提高牛呼吸道先天免疫力。本研究为研发口蹄疫灭活病毒的鼻腔疫苗提供重要的试验数据和参考。To explore the influence of innate immunity on cattle respiratory tract after intranasal aerosol immunized with inactivated foot-and-mouth disease virus （FMDV） along with Bacillus subtilis. In this study, we used inactivated FMDV along with B.subtilis to immune cattle by intranasal aerosol, and set up control group including single use of B.subtilis, inactivated FMDV,conventional inactivated FMDV vaccine and negative control. The results of immunohistochemistry assay showed that expressing TLR7 and CD3＋ lymphocytes were significantly increased in cattle nasal mucosae and pulmonary bronchial （p〈0.01） after intranasal immunized with inactivated FMDV along with B.subtilis, but there was no significant influences （p〉0.05） in the cattle only immunized with conventional inactivated FMDV vaccine compared with other control group. At the same time, the results of ELISA showed that the level of IL-12 was significantly increased （p〈0.01, p〈0.05） and the level of IL-4 and IL-10 were not significant （p〉0.05） in cattle respiratory tract tissues after intranasal immunized with inactivated FMDV along with B.subtilis, included nasal, tracheal, pulmonary and pulmonary bronchial. However, the level of IL-4, IL-10 and IL-12 were not significant （p〉0.05） in cattle respiratory tract tissues after the inactivated FMDV vaccine immunization. These results indicated that innate immunity of the cattle respiratory tract were improved by intranasal immunization of inactivated FMDV along with B.subtilis. Our study provided important experimental data and reference for the development of the inactivated FMDV vaccine via intranasal aerosol immunization.

关 键 词：O型口蹄疫灭活病毒 枯草芽孢杆菌 鼻腔免疫 呼吸道黏膜 TLR7 CD3^+淋巴细胞 细胞因子 

分 类 号：S852.65[农业科学—基础兽医学]