小尾寒羊FSHR基因在生殖轴的表达研究  

Expression of FSHR Gene in Reproductive Axis of Small-tail Han Sheep

作　　者：寸静宇 刘秋月[1] 王翔宇[1] 狄冉[1] 胡文萍[1] 张效生[3] 张金龙[3] 赵永聚[2] 储明星[1] Cun Jingyu;Liu Qiuyue;Wang Xiangyu;Di Ran;Hu Wenping;Zhang Xiaosheng;Zhang Jinlong;ZhaoYongju;Chu Mingxing(Key Laboratory of Animal Genetics,Breeding and Reproduction of Ministry of Agriculture,Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100193,China;College of Animal Science and Technology,Southwest University,Chongqing 400715,China;Tianjin Institute of Animal Sciences,Tianjin 300381,China)

机构地区：[1]中国农业科学院北京畜牧兽医研究所农业部动物遗传育种与繁殖重点实验室,北京100193 [2]西南大学动物科技学院,重庆400715 [3]天津市畜牧兽医研究所,天津300381

出　　处：《中国草食动物科学》2018年第6期13-15,57,共4页China Herbivore Science

基　　金：国家自然科学基金项目(31772580;31772564);国家转基因科技重大专项(2016ZX08009-003-006;2016ZX08010-005-003);国家肉羊产业技术体系专项(CARS-38);中央级公益性科研院所基本科研业务费专项(Y2017JC24);中国农业科学院科技创新工程(ASTIP-IAS13;CAAS-XTCX2016010-01-03;CAAS-XTCX2016010-03-03;CAAS-XTCX2016011-02-02);宁夏农林科学院科技创新先导资金项目(DWJLC-2016001);内蒙古自治区科技重大专项;农业科研杰出人才及其创新团队项目(农办人[2015]62号);国家万人计划科技创新领军人才项目(W02020274);天津市农业科技成果转化与推广项目(201704020);重庆高校创新团队建设计划资助项目(CXTDG201602004);重庆市社会民生科技创新专项项目(cstc2017shms-zdyfX0045)

摘　　要：为了揭示FSHR基因在小尾寒羊下丘脑-垂体-卵巢轴(Hypothalamic-pituitary-ovarian axis,HPOA)中的表达规律,深入了解其对小尾寒羊多羔的作用,采用实时荧光定量PCR技术对6只小尾寒羊(FecB++型单、多羔母羊各3只)的生殖组织及脑组织中FSHR基因的表达谱进行分析。结果显示:FSHR基因在大脑、卵巢和垂体组织表达,其中在卵巢高表达;FSHR基因在小尾寒羊多羔群体卵巢和垂体的表达量均极显著高于单羔群体(P<0.01)。研究发现,FSHR基因的表达水平与小尾寒羊产羔数之间存在一定程度相关,暗示其可能参与了小尾寒羊多羔性状调控。In order to reveal the expression patterns of FSHR gene in the hypothalamic-pituitary-ovarian axis（HPOA）in Small-tail han sheep,and further research the effect of FSHR gene in prolificacy,the real-time fluorescence quantitative PCR（RT-qPCR） method was used to detect the expression of the FSHR gene in reproductive tissues and brain tissues of six Small-tail han sheep（three polytocous and three monotocous ewes from the FecB＋＋ genotype） in this study. The results showed that FSHR gene expressed in the brain,ovary and pituitary tissues,FSHR was highly expressed in ovary. The expression of FSHR gene in ovary and pituitary of polytocous Small-tail han ewes was extremely significantly higher（P〈0.01） than that of monotocous ewes. The preliminary results of this study showed that there was a positive correlation between the expression level of FSHR gene and the litter size of Small-tail han sheep,so,the FSHR gene maybe involve in the regulation of multiple lambs in Small-tail han sheep.

关 键 词：小尾寒羊 多羔 FSHR基因 组织表达 

分 类 号：S826.2[农业科学—畜牧学]