野生东方田鼠4种成纤维细胞培养上清对日本血吸虫童虫的体外杀伤效果  

Killing Effect Investigation to Schistosomula through In Vitro Co-Culturing with Four Kinds of Fibroblasts Culture Supernatant from Microtus fortis

作　　者：吴侠 成钢 黎欢 李海霞 汤志宏 WU Xia;CHENG Gang;LI Huan;LI Hai-xia;TANG Zhi-hong(Air Command outpatient department of Military Region,Guangzhou 510071,China;Institute of animal health culture Hunan University of Arts and Science Chang de 415000,China)

机构地区：[1]南部战区空军参谋部门诊部妇儿科,广州510071 [2]湖南文理学院生命与环境科学学院动物健康养殖研究所,常德415000

出　　处：《实验动物与比较医学》2018年第5期372-376,共5页Laboratory Animal and Comparative Medicine

基　　金：湖南文理学院大学生创新创业研究项目(ZC1626; YB1719);湖南文理学院芙蓉学院大学生研究性学习与创新性实验计划项目(YB1703)

摘　　要：目的为了明确野生东方田鼠4种成纤维细胞培养上清对体外培养的日本血吸虫童虫的杀伤效果。方法以对数生长期的野生东方田鼠胚胎、皮肤、肺脏及腹腔成纤维细胞培养上清液(实验组)与血吸虫童虫共培养,以DMEM培养基为空白对照,以含体积分数20%的东方田鼠血清为阳性对照,96 h内观察与4种细胞培养上清共培养的童虫状态,统计死亡率。结果与野生东方田鼠腹腔与肺脏成纤维细胞培养上清共培养72 h后,童虫出现死亡特征,表现为表膜皱缩,缢痕明显; 96 h后,虫体内容物模糊并溢出,与阳性对照组童虫死亡特征略有不同;野生东方田鼠皮肤、胚胎、腹腔和肺脏组织成纤维细胞培养上清共培养96 h的血吸虫童虫死亡率分别为8.30%±2.10%、9.30%±1.38%、17.20%±3.27%和15.10%±4.35%,与空白对照的童虫死亡率(7.70%±0.80%)相比表明,腹腔和肺脏成纤维细胞培养上清液对童虫具有明显杀伤效果(P<0.05)。结论对数生长期的野生东方田鼠腹腔和肺脏成纤维细胞培养上清对日本血吸虫童虫的杀伤效果较明显。Objective To identify the kill effect of Microtus fortis culture supernatants against Schistosoma japonicum. Method Co-culture schistosomula with supernatants of wild Microtus fortis embryos, skin, lungs and peritoneal fibroblasts were collected at the logarithmic phase in this study. The death rate of schistosomula was observed and calculated in 96 h. We used 20% M. fortis serum as a positive control and DMEM medium as a blank control. Result After co-culture with lungs and peritoneal fibroblasts culture supernatant 72 h, schistosomula show death sign, including： shrinking skin,constriction. After 96 h, schistosomula dissolved, and the death state of the schistosomula was slightly different with the positive control group. The death rate is 8.3% ± 2.1%, 9.3% ± 1.38%, 17.2%± 3.27% and 15.1% ± 4.35% respectively after 96 h co-culture with Microtus fortis skin, embryos,peritoneal fibroblasts and lungs. Conclusion Killing effect of lungs and peritoneal fibroblasts culture supernatant of M. fortis was higher than that of the same treated skin and embryonic cell group in vitro（P〈0.05）.

关 键 词：东方田鼠 日本血吸虫 成纤维细胞 童虫 杀伤作用 

分 类 号：R532.21[医药卫生—内科学] Q95-33[医药卫生—临床医学]