温水浴复温对长时程海水浸泡体温过低大鼠血液生化及凝血的影响  

Effects of warm water bath rewarming on blood biochemistry and coagulation in rats with hypothermia induced by prolonged seawater immersion

作　　者：乔媛媛[1] 史成和[1] 奂剑波 陈丽娜 宋立成 程浩[1] 张达矜[1] 韩志海 Qiao Yuanyuan;Shi Chenghe;Huan Jianbo;Chen Lina;Song Licheng;Cheng Hao;Zhang Dajin;Han Zhihai(Naval Combat Casualty Care Research Center,Navy General Hospital,Beijing 100048,China)

机构地区：[1]海军总医院海战伤救治研究中心,北京100048 [2]海军总医院呼吸与危重症医学科 [3]解放军医学院研究生院 [4]安徽医科大学研究生院

出　　处：《中华航海医学与高气压医学杂志》2018年第5期273-280,共8页Chinese Journal of Nautical Medicine and Hyperbaric Medicine

基　　金：军队后勤科研计划重点项目（BHJ14C009）;军队后勤科研计划重点项目（BHJ16J011）;军队医学科技青年培育项目（16QNP023）

摘　　要：目的观察长时程海水浸泡体温过低症SD大鼠温水浴复温前后血液生化和凝血功能的变化。方法100只雄性成年SD大鼠随机均分为正常对照组（NC组，不做任何处理），低温实验组（HT组，20 ℃海水浸泡24 h），被动复温（pR）1、2、3、4组（20 ℃海水浸泡24 h＋被动复温，分别在复温后0、3、6、12 h处死），温水浴复温（wR）1、2、3、4组（20 ℃海水浸泡24 h＋温水浴复温，分别在复温后0、3、6、12 h处死），每组10只。腹主动脉抽血，检测重要的血液生化和凝血功能指标。结果长时程海水浸泡体温过低症SD大鼠在温水浴复温后0～3 h，血液肝功、肾功、心肌酶重要指标维持高水平，甚至有所上升，在复温6 h后才显著下降；凝血功能指标在复温后0 h即开始出现下降。与被动复温组相比，温水浴复温组改善肝功、肾功、心肌酶指标异常的作用更好，差异有统计学意义（P〈0.05）。结论温水浴复温对长时程海水浸泡体温过低症大鼠血液生化指标异常的恢复效果好于被动复温。Objective To observe changes in blood biochemistry and coagulation function before and after warm water bath rewarming in SD rats with hypothermia induced by prolonged seawater immersion. Methods One hundred male Sprague-Dawley rats were randomly divided into the normal control group （the NC group, without any treatment） and the hypothermia group （the HT group, exposed to seawater immersion at 20 ℃ for 24 hours）. The passive rewarming sub-groups （the pR group 1, 2, 3 and 4, n=10） （exposed to seawater immersion at 20 ℃ for 24 hours ＋ passive rewarming） were respectively executed after rewarming at hour 0, hour 3, hour 6, and hour 12）. The warm-water bath rewarming sub-groups （the wR group 1, 2, 3 and 4, n=10） （exposed to seawater immersion at 20 ℃ for 24 hours＋ warm-water bath rewarming） were respectively executed after rewarming at hour 0, hour 3, hour 6, and hour 12）. Blood samples were taken from abdominal aorta for biochemical and coagulation detection.Results Zero to 3 hours after warm water bath rewarming, important parameters of hepatic and renal functions, as well as myocardial enzymes in the SD rats with hypothermia induced by prolonged seawater immersion remained at high levels or even increased to some extent. Only after 6 hours after rewarming, the above-mentioned parameters dropped considerably. However, coagulation indicators began to decline immediately after rewarming, and as compared with the passive rewarming, warm water bath rewarming could produce obviously better effects on the alleviation of abnormal hepatic and renal functions, as well as myocardial enzyme parameters （P〈0.05）.Conclusion Warm water bath rewarming could produce better effects on the recovery of serum biochemical parameters in rats with hypothermia induced by prolonged seawater immersion when compared with those of passive rewarming.

关 键 词：海水浸泡 体温过低症 SD大鼠 温水浴复温 被动复温 血液生化 凝血 

分 类 号：R83[医药卫生—航空、航天与航海医学]