南京市6774份HIV抗体初筛阳性标本复检与确证结果分析  

Analysis of reexamination for HIV antibody positive specimens in Nanjing city from 2012 to 2017

作　　者：吴咏梅[1] 许文炯[1] 董潇潇[1] 董晓庆[1] 王燕[1] WU Yongmei;XU Wenjiong;DONG Xiaoxiao;DONG Xiaoqing;WANG Yan(Nanjing Center for Disease Control and Prevention,Nanjing 210003,China)

机构地区：[1]南京市疾病预防控制中心,南京210003

出　　处：《中国麻风皮肤病杂志》2018年第10期606-608,共3页China Journal of Leprosy and Skin Diseases

基　　金：江苏省卫生计生委2014-2015年度预防医学科研课题(编号:Y2015002)

摘　　要：目的:分析南京市2012-2017年6774份HIV抗体初筛、复检与确证实验的准确性。方法:对2012年1月至2017年12月该市艾滋病筛查实验室初筛阳性送检样本6774份,采用两种酶标法和金标法进行复检,复检阳性可疑标本进行免疫印迹法(WB)确证检测。结果:6774份初筛阳性标本经金标和酶标复检,复检双阳4821份,其中确证阳性4736份,确证阴性3份,不确定82份。金标和酶标复检单阳97份,其中确证阴性51份,阳性3份,不确定43份。2012-2017年HIV不确定率分别为1.5%,1.8%,1.9%,2.2%,2.3%,2.6%。结论:HIV初筛假阳性增多现象应引起重视;随着HIV不确定率逐年增多,需要找到快速鉴别HIV抗体的方法。Objective： To analyze the accuracy of HIV antibody testing with different tests in Nanjing from2012 to 2017. Methods： Six thousand seven hundred and seventy-four samples which were positive with primary screening test for HIV were reexamined through ELISA and JB-P reagent. And then,the reexamined positive specimens were confirmed by Western blot（ WB）. Results： Of 6774 samples,4821 were both positive with ELISA and JB-P reagent. Of 4821 samples,4736 were positive with WB,3 were negative and 82 were uncertained. Out of 97 positive samples tested with ELISA or JB-P,3 were positive with WB,51 were negative and 43 were uncertained. The uncertainty rates from 2012 to 2017 were 1.5%,1.8%,1.9%,2.2%,2.3% and 2.6%. Conclusion： The increase in the number of false positivity in screening laboratories should be concrened. As uncertainty rate is increasing year by year,it is necessary to develop new HIV tests for the identification of HIV infections.

关 键 词：HIV抗体 初筛阳性 复检 

分 类 号：R512.91[医药卫生—内科学]