氧化苦参碱对转化生长因子β1诱导大鼠心肌成纤维细胞增殖与分化的抑制作用  

Oxymatrine Has Inhibitory Effect on Proliferation and Differentiation of Cardial Fibroblasts in Rats Induced by Transforming Growth Factor-β1

作　　者：乔璐[1] 李博[1] 李庆[1] QIAO Lu;LI Bo;LI Qing

机构地区：[1]郑州市第一人民医院,河南郑州450008

出　　处：《新中医》2018年第11期6-9,共4页New Chinese Medicine

基　　金：河南省科技攻关计划项目(162300410252)

摘　　要：目的:观察氧化苦参碱(Oxymatrine,OM)干预对转化生长因子β1 (Transforming growth factor-β1,TGF-β1)刺激的SD大鼠心肌成纤维细胞(Cardialfibroblasts,CFs)增殖、分化的抑制作用,并探讨其作用机制。方法:体外培养新生大鼠CFs,分离纯化后分为对照组(使用无血清DMEM培养)、TGF-β1组(单纯TGF-β1诱导)和观察组(使用TGF-β1加OM干预) 3组。MTT法检测CFs增殖情况,ELISA法检测Ⅰ型胶原(Collagen-Ⅰ,CoL-Ⅰ)和Ⅲ型胶原(Collagen-Ⅲ,CoL-Ⅲ)含量,RT-PCR法检测Smad2 mRNA表达,Westorn blot法检测增殖细胞核抗原(PCNA)、α-平滑肌肌动蛋白(α-SMA)、Smad2及Smad3蛋白表达情况。结果:与对照组比较,TGF-β1组CFs增殖水平、CoL-Ⅰ、CoL-Ⅲ、PCNA、α-SMA、Smad2 mRNA及Smad2/3蛋白表达均明显升高(P <0.05);与TGF-β1组比较,观察组上述指标均降低(P <0.05),除CoL-Ⅰ外,差异均有统计学意义(P <0.05)。结论:OM能够明显抑制TGF-β1诱导CFs增殖、分化水平,降低胶原蛋白的合成水平,作用机制与其能够干预Smad2及Smad3蛋白表达有关,具有临床应用价值。Objective： To observe the inhibitory effect of oxymatrine（OM） on the proliferation and differentiation of cardial fibroblasts （CFs） in SD rats induced by transforming growth factor-β1（TGF-β1） and explore its mechanism. Methods： Separated and purified CFs of neonatal rats in vitro, and divided them into the control group（cultured by serum-free DMEM）, the TGF-β1 group（simply induced by TGF-β1） and the observation group（intervened by TGF-β1 and OM）. Detected the proliferation of CFs by MTT method, the content of collagen-I（CoL-I） and collagen- III （COL- III） by ELISA method and the expression of mRNA of Smad2 by RT-PCR method. Detected the expressions of proliferating cell nuclear antigen（PCNA）, α-smooth muscle actin （α-SMA） and protein expressions of Smad2 and Smad3 by Westorn blot method. Results： Compared with those in the control group, the proliferation of CFs and the expressions of CoL-I, CoL-III , PCNA, α-SMA and protein expressions of Smad2 Smad2/3 in the TGF-β1 group were obviously increased（P 〈 0.05）. Compared with those in the TGF-β1 group, the above indexes（except CoL-I）in the observation group were decreased, differences were significant （P 〈 0.05）. Conclusion- OM can obviously inhibit the levels of proliferation and differentiation of CFs induced by TGF-β1 and decrease the translation level of collagen. Its mechanism is related to the intervention of the protein expressions of Smad2 and Smad3, which maintains high value in clinical practice.

关 键 词：氧化苦参碱(OM) 心肌成纤维细胞(CFs) 抑制作用 转化生长因子β1(TGF-β1) 动物实验 大鼠 

分 类 号：R285.5[医药卫生—中药学]