丹蛭降糖胶囊对糖尿病动脉硬化模型大鼠胸主动脉p22phox mRNA、p47phox mRNA表达的影响  

Danzhi Jiangtang Capsules Has Effect on the Expression of p22phox mRNA and p47phox mRNA of Thoracic Aorta in Model Rats with Diabetic Arterosclerosis

作　　者：罗云[1] 方朝晖[2] LUO Yun;FANG Zhaohui

机构地区：[1]杭州市余杭区中医院,浙江杭州311106 [2]安徽中医药大学第一附属医院,安徽合肥230031

出　　处：《新中医》2018年第8期5-8,共4页New Chinese Medicine

基　　金：国家中医药管理局中医药重点学科项目(20091221);国家科技部十二五科技支撑计划新安医学传承与发展研究项目(2012BA126B00)

摘　　要：目的:探讨益气养阴活血方丹蛭降糖胶囊(DJC)对糖尿病大血管病变的作用机制。方法:SD雄性大鼠60只,适应性喂养1周,随机分为空白对照组10只与造模组50只。空白对照组予普通饲料喂养。造模组按70万IU/kg的总剂量灌胃维生素D3,分3天给完,同时高脂饲料喂养,诱发快速动脉硬化的大鼠模型,4周以后,采用35 mg/kg链脲佐菌素(STZ)腹腔注射,诱发糖尿病合并动脉硬化大鼠模型,最终造模成功的大鼠随机分为模型组,DJC低、中、高剂量组,分别予以相应药物灌胃,疗程12周。检测治疗前后大鼠空腹血糖(FBG)、胸主动脉p22phox mRNA及p47phox mRNA的表达。结果:与空白对照组比较,模型组大鼠治疗前后FBG水平显著升高,胸主动脉p22phox mRNA、p47phox mRNA表达水平升高,差异均有统计学意义(P<0.01)。与治疗前比较,DJC中、高剂量组大鼠FBG降低,差异有统计学意义(P<0.01)。与模型组比较,DJC中、高剂量组大鼠FBG水平降低,胸主动脉p22phox mRNA、p47phox mRNA表达降低,差异均有统计学意义(P<0.01)。与DJC低剂量组比较,DJC中、高剂量组大鼠FBG水平较低,胸主动脉p22phox mRNA、p47phox mRNA表达较低,差异均有统计学意义(P<0.05)。结论:丹蛭降糖胶囊能够降低糖尿病大鼠FBG,降低胸主动脉p22phox mRNA和p47phox mRNA的表达,抑制氧化应激,从而有效防治和延缓糖尿病大血管病变。Objective： To explore the mechanism of Yiqi Yangyin Huoxue prescription and Danzhi Jiangtang capsules（DJC） on diabetic macroangiopathy. Methods：After conducting adaptive feeding to 60 cases of male SD rats for one week,divided them into the blank control group and the modeling group randomly,10 cases and 50 cases in each group respectively. The blank control group was fed with normal diet. The modeling group was given vitamin D3 with a total dose of 700,000 IU/kg by gavage in 3 days and was fed with high fat diet meanwhile to induce and establish rat models with rapid arteriosclerosis. After 4 weeks,35 mg/kg STZ intraperitoneal injection was used to induce and establish rat models with diabetes and arteriosclerosis. Those rats with successful modeling were randomly divided into the model group,the DJC groups of low,middle and high dose,and were given corresponding drugs by gavage respectively for a course of 12 weeks. Detected the expression of fasting blood glucose（FBG） and p22 phox m RNA and p47 phox m RNA of thoracic aorta in rats before and after treatment. Results： Compared with that in the blank control group, the level of FBG in the model group was increased more significantly before and after treatment,and expression levels of p22 phox m RNA and p47 phox m RNA of thoracic aorta were increased,differences being significant（P〈0.01）. Levels of FBG in the DJC groups of middle and high dose were decreased when compared with those before treatment, the difference being significant（P〈0.01）. Compared with those in the model group,the levels of FBG and expression levels of p22 phox m RNA and p47 phox m RNA of thoracic aorta in the DJC groups of middle and high dose were decreased,differences being significant（P〈0.01）. Compared with those in the DJC group of low dose,levels of FBG and expression levels of p22 phox m RNA and p47 phox m RNA of thoracic aorta in the DJC groups of middle and high dose were lower, differences being significant（P〈0.05）. Conclusion： Danzhi Jiangtang

关 键 词：糖尿病大血管并发症 丹蛭降糖胶囊 益气养阴活血 P22PHOX MRNA p47phox MRNA 动物实验 大鼠 

分 类 号：R587.2[医药卫生—内分泌]