葛花总黄酮对异丙肾上腺素致大鼠内耳损伤后相关炎性因子影响的实验研究  

Experimental study on the effect of total flavonoids of Pueraria lobata on inflammatory cytokines in rats with inner ear injury induced by isoproterenol

作　　者：赵江涛[1] 梁延鸽 ZHAO Jiangtao;LIANG Yange(Gansu Provincial Hospital of Chinese Medicine,Lanzhou 730050,Gansu,China;Jinan University,Jinan 250022,Shandong,China)

机构地区：[1]甘肃省中医院 [2]济南大学

出　　处：《中国临床药理学与治疗学》2018年第9期1003-1007,共5页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：甘肃省中医药管理局科研课题项目(GZK-2016-38);国家自然科学基金项目(30701022)

摘　　要：目的:探究中药葛花总黄酮对异丙肾上腺素致大鼠内耳损伤后相关炎性因子的影响机制。方法:将大鼠分为4组,葛花总黄酮高、低剂量组,模型组及阴性组,探讨预防给药后对异丙肾上腺素致内耳损伤大鼠的TNF-α、IL-4,ACTH及Bax蛋白含量的变化。结果:造模后,与阴性组对比,给药组和模型组血清TNF-α、IL-4、ACTH、Bax含量明显升高(P <0. 05);与模型组对比,给药组血清TNF-α、IL-4、Bax含量降低(P <0. 05),ACTH含量升高(P <0. 05),其中高剂量组比低剂量组变化更加明显(P <0. 01)。结论:葛花总黄酮对异丙肾上腺素致大鼠内耳损伤能够有效抑制TNF-α、IL-4炎症因子的释放,调节ACTH、Bax蛋白的表达。AIM： To investigate the effect of to- tal flavonoids of Pueraria lobata on inflammatory factors after isoprenaline induced inner ear injury in rats. METHODS： The rats were divided into four groups： the high and low dose group of total flavonoids of Pueraria lobata, the model group and the negative group. The changes of TNF-α, IL-4, ACTH and Bax protein in rats with inner ear injury induced by isoproterenol were observed and compared. RESULTS： Compared with the negative group, the serum TNF-ot, IL-4, ACTHX, Bax content in the administration group and the model group was significantly higher than that in the model group （ P 〈 0. 05 ）, and was significantly higher than thosein the model group （P 〈0.05）. The content of TNF-α, IL-4, Bax in serum decreased （P 〈 0.05） and the content of ACTH increased in the treatment group （P 〈 0.05 ）, especially in the high-dose group compared with the low-dose group （P 〈 0.01 ）. CONCLUSION： Total flavonoids of Pueraria lobata can effectively inhibit the release of TNF- α, IL-4 inflammatory factor and regulate the expression of ACTH, Bax protein in rat inner ear injury induced by isoprenaline.

关 键 词：葛花总黄酮 内耳损伤 TNF-Α IL-4 ACTH BAX 

分 类 号：R965.2[医药卫生—药理学]