白杨素减轻人脐静脉内皮细胞的氧化应激损伤  

Chrysin attenuates oxidative stress in human umbilical vein endothelial cells

作　　者：高雪梅 张夏丽[2] 陈敏[2] 李洁[2] 罗乐[2] 韩斯嘉 董季 宋振蓉 张轩萍[2] GAO Xuemei;ZHANG Xiali;CHEN Min;LI jie;LUO Le;HAN Sijia;DONG ji;SONG Zhenrong;ZHANG Xuanping(Department of Physiology,Shanxi Health Vocational College,Jinzhong 030619,Shanxi,China;Department of Pharmacology,Shanxi Medical University,Taiyuan 030001,Shanxi,China)

机构地区：[1]山西卫生健康职业学院生理学教研室 [2]山西医科大学药理学教研室

出　　处：《中国临床药理学与治疗学》2018年第9期1022-1026,共5页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：山西省卫生计生委科研项目(2015109);山西省自然科学基金面上项目(201601D102073)

摘　　要：目的:观察白杨素对人脐静脉内皮细胞(human umbilical vein endothelial cells,HUVEC)氧化应激损伤的保护作用。方法:常规培养HUVEC细胞,分为5组:对照组,不加任何干预;H2O2组,用400μmol/L过氧化氢处理2 h;白杨素高、中、低剂量组,提前用100、50、25μmol/L的白杨素孵育12 h,然后用400μmol/L过氧化氢处理2 h。处理结束后,用MTT法检测活细胞数目,并检测乳酸脱氢酶(LDH)的漏出。收集细胞上清液及细胞裂解液,检测NO、丙二醛(MDA)产量及内皮型一氧化氮合酶(eNOS)、超氧化物歧化酶(SOD)活性。用荧光染料DCEF-DA染色后,酶标仪检测活性氧(ROS)的产量。用荧光定量RTPCR的方法检测黏附分子的表达。结果:过氧化氢导致内皮细胞活细胞数目减少,LDH漏出增多,MDA、ROS产生增多,SOD活性下降,黏附分子表达增多,内皮细胞NO产生减少,eNOS活性下降。提前用白杨素预孵,可以增加细胞存活率,减轻氧化应激损伤,减少黏附分子的表达,eNOS蛋白活性增加,细胞NO的产量增加。结论:白杨素可以减轻HUVEC的氧化应激损伤,减少黏附分子的表达,增加细胞NO的合成能力。AIM： To observe the protective effects of chrysin against H202-induced oxidative stress injury in human umbilical vein endothelial cells （HUVECs） and the possible mechanism. METHODS： HUVECs were subjected to H202 in the absence or presence of chrysin （25, 50, 100 μmol/L） for 2 h. The 3-（4,5-Dimethyhhiazol-2-yl 1） -2,5 -diphenyltetrazolium bromide （MTT） assay was used to assess cell viability. Reactive oxygen species was detected by dichlorofluorescein diaeetate （DCFH-DA）. The release of lactate dehydrogenase （ LDH ）, the level of malondialdehyde （ MDA ）, NO, superoxide dismutase （SOD） and endothelial nitric oxide synthase （eNOS） in culture media or in cells were assessed using commercial kit according to the manufacturer＇s instructions. The expressions of adhesion molecule mRNA were detected by reversetranscription-quantitative polymerase chain reaction. RESULTS：The results showed that H202 increased the release of LDH, the production of MDA and ROS, the mRNA level of adhesion molecules, decreased the cell viability and the activity of SOD and eNOS, reduced the production of NO. Pretreating HUVECs with chrysin improved cell viability and inhibited the release of LDH, reduced the production of ROS and MDA, alleviated the mRNA level of ad- hesion molecules, elevated the production of NO, improved the activity of SOD and eNOS. CONCLUSION： Chrysin might exert protective effects against oxidative stress in HUVEC.

关 键 词：白杨素 人脐静脉内皮细胞 氧化应激 

分 类 号：R965.2[医药卫生—药理学]