不同激活剂对人富血小板血浆释放曲线的影响  

The effects of different activators on the release curve of human platelet- rich plasma

作　　者：庄雅雯 曾奕明[1] 陈云峰[1] 张华平[1] 陈晓阳[1] 扬栋勇 吴炜景[1] Zhuang Yawen;Zeng Yiming;Chen Yunfeng;Zhang Huaping;Chen Xiaoynng;Yang Dongyong;Wu Weijing.(Department of Pulmonary and Critical Care Medicine,Second Affiliated Hospital of Fujian Medical University,Respiratory Medicine Center of Fujian province,Quanzhou 362000,China)

机构地区：[1]福建医科大学附属第二医院呼吸与危重症医学科福建省呼吸医学中心,泉州362000

出　　处：《中华结核和呼吸杂志》2018年第11期868-872,共5页Chinese Journal of Tuberculosis and Respiratory Diseases

摘　　要：目的对比分析不同激活剂对富血小板血浆（PRP）中转化生长因子．B，（TGF-13．）及血小板源性生长因子（PDGF-AB）释放曲线的影响。方法抽取10名健康成年志愿者外周静脉全血36m1，采用二次离心法自制PRP；按1000U凝血酶溶于1ml10％氯化钙溶液制备血小板激活剂。按10：1的容量比将PRP与激活剂混合（凝血酶-PRP组）；按10：1的容量比将PRP与10％氯化钙混合（氯化钙-PRP组1；分别以新鲜全血（全血组）以及未加血小板激活剂的PRP（PRP组）为对照组，将上述4组在37℃温水中孵育0、1、8、24、72和168h，采用ELISA法测定各组不同时间点的TGF-B．和PDGF．AB浓度，绘制TGF-β1和PDGF．AB的释放曲线，并采用重复测量方差分析比较不同组别中TGF-B，和PDGF．AB的释放曲线。结果（I）全血组与PRP组中TGF．B．和PDGF-AB在168h内随着时间的推移而持续增高。PRP在与凝血酶混合后，立即形成凝胶状，TGF-β1，和PDGF-AB均立即明显升高，在激活后1h达到高峰，TGF-β1，和PDGF-AB分别由（42±21）和（77±18）μg／L增至高峰（84±21）和（124±35）μg／L，随后逐渐下降，释放曲线直接而快速；而PRP与氯化钙混合后，约1h才形成凝胶状，TGF-β．和PDGF-AB均缓慢持续增高，可在168h内-直保持较高水平。（2）PRP组中TGF-B．和PDGF-AB的AUC0-168h均高于全血组（均P〈0.05），氯化钙-PRP组中TGF-β1，的AUC0-168h高于凝血酶．PRP组（Z=-2．26，P〈0．05），但氯化钙-PRP组与凝血酶-PRP组中PDGF-AB的AUC0-168h比较差异无统计学意义（Z=-1．512，P=0．131）。结论使用氯化钙作为激活齐lj时富血小板血浆中TGF-B、和PDGF-AB释放浓度较高，释放时间较长，曲线下面积最大。Objective To compare and analyze the effects of different activators on the release curve of TGF-β1 and PDGF-AB in platelet rich plasma（PRP）. Methods A total of 36 ml peripheral venous blood was obtained from 10 healthy adult volunteers, and the PRP was made by secondary centrilugation. The platelet activator was made by bovine thrombin l 000 U in 1 ml 10% calcium chloride solution. The Thrombin-PRP group was made by PRP and the activator in a ratio of 10：1 .The Calcium chloride-PRP group was made in a ratio of 10：1 by PRP and 10% calcium chloride solution instead. The fresh whole blood（whole blood group） and inaetived PRP（PRP group） were used as the control groups. The 4 groups were incubated in warm water of 37 ℃ for 0, 1, 8, 24,72 and 168 h. A quantitative sandwich enzyme-linked immunosorbent assays（ELISA） was used to examine the amount of TGF-β1 and PDGF-AB in different time points of each group. The release curves of TGF- β1 and PDGF- AB were based on afore- mentioned data, and then comparisons of the release curves of TGF-β1 and PDGF-AB in different groups were peffm3ned by repeated measurement variance analysis. Results （1）The levels of TGF-β1 and PDGF-AB in the whole blood group and the PRP group continued to increase within 168 h. PRP immediately formed into a gel after mixture with thrombin combined and calcimn chloride, and the concentrations of TGF-β1 and PDGF-AB reached the peak in 1 h after activation; increased from （42±21）ng/ml and （77±18）ng/m1 to （84±21）ng/ml and （124±35）ng/ml, respectively, and then decreased gradually. The release curve was direct and rapid. The PRP became a gel state in approximate 1 h after mixture with calcium chloride, and the concentrations of TGF-β1 and PDGF- AB were slowly rising and remained high at 168 h.（2）The AUCo_16sh of TGF-β1 and PDGF-AB in the PRP group was higher than that in the whole blood group （all P〈0.05 ）, and the AUC0-1681, of TGF-β1 in the Calcium chloride-PRP group was higher than that in

关 键 词：富血小板血浆 酶激活 血小板源性生长因子 转化生长因子Β1 

分 类 号：R457.14[医药卫生—治疗学]