饥饿对高体革鯻消化道黏液细胞分布的影响  

Effects on Mucous Cells Distribution in Digestive Tract of Scortum barcoo

作　　者：谢木娇 钟金香 颉晓勇[1] 朱长波[1] 李俊伟[1] 王 叶国玲 鲍虞园 Xie Mujiao;Zhong Jinxiang;Xie Xiaoyong;Zhu Changbo;Li Junwei;Wang Jun;Ye Guoling;Bao Yuyuan(Key Laboratory of South China Sea Fishery Resources Exploitation ＆ Utilization,Ministry of Agriculture-and Rural Affairs,Guangdong Provincial Key Laboratory of Fishery Ecology and Environment,South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangzhou 510300,China;Guangdong Provincial Ocean and Fishery Technical Extension Center,Guangzhou 510222,China;College of Fisheries,Tianjin Agricultural University,Tianjin 300384,China;College of Fisheries and Life Sciences,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]中国水产科学研究院南海水产研究所农业农村部南海渔业资源开发利用重点实验室广东省渔业生态环境重点实验室,广州510300 [2]广东省海洋与渔业技术推广总站,广州510222 [3]天津农学院水产学院,天津300384 [4]上海海洋大学水产与生命学院,上海201306

出　　处：《中国细胞生物学学报》2018年第10期1685-1691,共7页Chinese Journal of Cell Biology

基　　金：广东省海洋渔业科技攻关与研发项目(批准号:A201601B14);国家青年科学基金(批准号:31302200)资助的课题~~

摘　　要：利用石蜡组织切片与AB-PAS染色技术研究饥饿0天(S0)、10天(S10)、20天(S20)对高体革消化道黏液细胞形态及分布的影响。结果发现,高体革饥饿20天后存活率为100%,食道含有Ⅱ、Ⅲ、Ⅳ型黏液细胞, S0组黏液细胞总密度为(2 839.13±261.52)个/mm2,饥饿不影响食道黏液细胞的种类,但S10组黏液细胞总密度减少[(1 546.00±70.02)个/mm2], S20组则表现为增加[(3 095.00±162.04)个/mm2]。S0、S10、S20三组胃部黏液细胞分布差异不大。S0和S10组幽门盲囊含Ⅰ和Ⅱ型黏液细胞, S20组则只有Ⅱ型黏液细胞,总密度先增加后减少,分别是(215.22±21.52)、(466.31±63.05)、(369.46±43.49)个/mm2。肠道只含Ⅱ型黏液细胞,饥饿使其密度逐渐增多,尤其是后肠,S0、S10、S20组密度分别是(1 683.50±219.64)、(2 068.71±65.38)、(4 622.50±85.60)个/mm2。不同部位黏液细胞对饥饿的响应存在差异,反映消化道不同部位结构功能的差异,饥饿显著影响消化道中Ⅱ型黏液细胞的分布,推测Ⅱ型黏液细胞分泌的变化有可能是高体革响应饥饿应激的敏感指标之一。We evaluated the effects of starvation on mucous cells features in digestive tract of Scortum barcoo. Fishes were deprived of feed for twenty days, sampled from first day （SO）, tenth day （S10） and twentieth day （S20） and the analysis of mucous cells in digestive tract were examined using AB-PAS （Alician blue and periodic acid Schiff reagent, Alician blue at pH2.5） staining technology. No death was found after the starvation. Type Ⅱ, Ⅲ, IV mucous cells were found in esophagus mucosa and starvation had no effect on the type of mu- cous cells, while impacted the density deeply, statistic showed that mucous cells densities in SO, S10, S20 were （2 839.13±261.52）, （1 546.00±70.02）, （3 095.00±162.04） cells/mm^2 respectively. In group SO and S10, type Ⅰ and Ⅱ mucous cells were found in pyloric ceeca, density increased from （215.22±21.52） cells/mm^2 to （466.31±63.05） cells/ mm^2, but decreased as （369.46＋43.49） cells/mm^2 with only type II mucous cells in groun S20. Type Ⅱ m.caus cells in intestine were increasing obviously after starvation, especially in hindgut, where the densities were （1 683.50±219.64）, （2 068.71±65.38）, （4 622.504±85.60） cells/mm^2 in group SO, S10, S20, respectively. The different responds of mucous cells distribution in different localization of digestive tract showed the difference structure and function in different position. With type II mucous cells changing amazingly, we speculated that the secretion of type II mucous cells could be used as a sensitive indicator to reflect starvation stress.

关 键 词：高体革鯻 饥饿 黏液细胞 

分 类 号：S917.4[农业科学—水产科学]