白三烯D4、白细胞介素6和肿瘤坏死因子α在成人分泌性中耳炎中耳积液中的表达及意义  

Expression and significance of LTD4, IL-6 and TNF-α in middle ear effusions in secretory otitis media

作　　者：马慧敏 马俭[1] MA Huimin;MA Jian(Department of Otolaryngology,Nanjing Hospital Affiliated to Nanjing Medical University,Nanjing,Jiangsu,210000,China)

机构地区：[1]南京医科大学附属南京医院耳鼻咽喉科,江苏南京210000

出　　处：《中国耳鼻咽喉头颈外科》2018年第10期530-532,共3页Chinese Archives of Otolaryngology-Head and Neck Surgery

摘　　要：目的探讨白三烯D4(leukotrieneD4,LTD4)、白细胞介素6(interleukin 6,IL-6)及肿瘤坏死因子α(tumour necrosis factorα,TNF-α)在成人分泌性中耳炎(secretory otitis media,SOM)患者中耳积液中的表达水平。方法采用酶联免疫吸附法测定32例(40耳)成人SOM患者中耳积液,其中28例患者(4例拒测)的外周血浆及20例健康人外周血浆中的LTD4、IL-6及TNF-α表达水平。结果 (1)LTD4、IL-6和TNF-α在所有标本中的检出率均为100%;(2)LTD4、IL-6在实验组表达显著高于对照组(t=2.436、2.596,P<0.05),TNF-α浓度表达与对照组比较,无统计学意义(P>0.05)。结论 LTD4和IL-6作为重要免疫介质,参与SOM的发生发展。OB J ECT I V E To investigate the expression and significance of LTD4, IL-6 and TNF-α in middle ear effusions in secretory otitis media（SOM） and their possible mechanism in the pathogenesis of SOM. METHODS Enzyme-linked immunosorbent assay were used to measured the expression levels of LTD4, IL-6 and TNF-α inmiddle ear effusions of 32 cases（40 ears） of adult SOM patients, peripheral blood plasma of 28 cases and 20 healthy human’s peripheral blood plasma. RESULTS1. The detection rates of LTD4, IL-6 and TNF-α were 100% in all samples. 2. The concentrations of LTD4 and IL-6 in the experimental group were significantly higher than those of the 2 control groups, and the results showed statistical significance（t =2.436 and 2.596, P〈0.05）. CONCLUSION LTD4 and IL-6 are involved in the immune mechanism of SOM, which arerelated to the developmentof inflammatory response in the tympanic cavity.

关 键 词：成年人 中耳炎 伴渗出液 白三烯D4 白细胞介素6 肿瘤坏死因子Α 

分 类 号：R764.21[医药卫生—耳鼻咽喉科]