基于荧光素酶报告基因的二英类生物检测法的发展与应用  

Development and application of luciferase reporter gene-based bioassay system for dioxin determination

作　　者：马慧 宋博宇[4] 张嵩岩 谢群慧[1,2] 徐丽 曹征宇[3] 赵斌 Hui Ma;Boyu Song;Songyan Zhang;Qunhui Xie;Li Xu;Zhengyu Cao;Bin Zhao(State Key Laboratory of Environmental Chemistry and Ecotoxicology,Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing 100085,China;University of Chinese Academy of Sciences,Beijing 100049,China;China Pharmaceutical University,Nanjing 211198,China;Foreign Economic Cooperation Office,Ministry of Envionmental Protection,Beo＇ing 100035)

机构地区：[1]环境化学与生态毒理学国家重点实验室中国科学院生态环境研究中心,北京100085 [2]中国科学院大学,北京100049 [3]中国药科大学,南京211198 [4]环境保护部环境保护对外合作中心,北京100035

出　　处：《中国科学：化学》2018年第10期1185-1194,共10页SCIENTIA SINICA Chimica

基　　金：国家自然科学基金(编号:21525730;21527901);中国科学院战略性先导项目(编号:XDB14030401);环保部环保公益类项目(编号:201309029);全球环境基金"中国制浆造纸行业二英减排项目(ID:4441)"资助

摘　　要：二英类生物检测法在大规模二英类筛查中具有重要的应用价值.其中,基于荧光素酶报告基因的二英类生物检测技术,是依据二英类化合物通过结合芳香烃受体从而引发一系列毒理效应的生物过程而设计的,具有灵敏度高、应用范围广、与毒理效应相关性强的特点.最新研究进展表明,该类技术可检测到的2,3,7,8-TCDD标准物质的最低浓度达到了0.1 pM,可应用于农业、食品、环境等领域的多种样品中痕量二英类化合物的筛查,在政府管理部门、第三方检测咨询机构及相关企业等方面具有广阔的应用和推广前景,同时,该方法在环境健康风险评估和相关基础研究中有更广阔的发展空间.本文着重介绍了基于荧光素酶报告基因的二英类生物检测法——CALUX生物检测法的原理、发展进程、应用现状和标准化的方法体系并对CALUX法的优缺点进行总结及之后的发展进行展望.Bioassay systems have been widely applied in large-scale screening of dioxins. Among these systems, the luciferase reporter gene-based bioassay system is designed based on aryl hydrocarbon receptor signaling pathway which mediates a series of toxicological effects of dioxins. The advantages of this bioassay system include high sensitivity, wide application and strong correlation with toxicological effects. Based on recent research on several newly developed systems, the minimum detection limit of such analytical method can reach 0.1 pM. This system could be widely used in agricultural, food, environmental, government administration and the third party inspection agency. Furthermore, a broad application prospect of this bioassay system has been proposed recently in environmental health study and health risk assessment. This review describes the development, application, standardized methods and mechanism of CALUX assay, as well as the future perspective.

关 键 词：二噁英 芳香烃受体 生物检测法 荧光素酶报告基因(CALUX) 

分 类 号：X830[环境科学与工程—环境工程]