7种杀菌剂对玉米大斑病病原菌的毒力测定试验  

Toxicity Test on Pathogen of Corn Northern Leaf Blight with Seven Fungicides

作　　者：王芳[1] 李桃 陈文胜[1] Wang Fang;Li Tao;Chen Wensheng(Foshan University of Science and Technology,Foshan Guangdong 52800)

机构地区：[1]佛山科学技术学院,广东佛山528000

出　　处：《中国农学通报》2018年第22期132-139,共8页Chinese Agricultural Science Bulletin

基　　金：国家级大学生创新创业训练计划项目"蔬菜几种主要真菌病害的药剂筛选"(XJ2016038)

摘　　要：研究旨在鉴定出玉米大斑病病原菌,筛选出防治玉米大斑病的最佳药剂。试验采用组织分离法对玉米大斑病病原菌进行分离鉴定,用含毒培养基生长速率法测定7种杀菌剂对玉米大斑病病原菌的抑制效果。经分离鉴定,该病原菌为链格孢Alternaria alternate。毒力测定试验结果表明:药后48 h,异菌脲EC50值为0.27 mg/L,毒力指数最大,对链格孢菌抑制效果非常显著;丙环唑和苯醚甲环唑EC50值为0.51、0.53 mg/L,抑制效果较显著;氟硅唑和嘧霉胺的EC50值为1.66、1.91 mg/L,效果显著;代森锰锌和醚菌酯的EC50值分别为25.82、929.78 mg/L,毒力指数较低,抑制效果差;药后72 h各药剂对链格孢的抑制效果与药后48 h抑制效果相似。在7种杀菌剂中异菌脲对链格孢菌抑制效果最好,当试验浓度为50 mg/L时,抑制率可达95%。The aim of this experiment is to identify the pathogen of corn northern leaf blight and screen out the best bactericide for controlling corn northern leaf blight. The tissue isolation method was used to isolate and identify the pathogen, and the growth rate method was used to determine the inhibitory effect of seven fungicides on pathogens of corn northern leaf blight. The pathogen was identified as Alternaria alternate, toxicity test results showed that： after 48 h, ECso of iprodione was 0.27 mg/L, the virulence index was the highest, and the inhibitory effect on Alternaria alternate was very significant. ECso of propiconazole and difenoconazole were 0.51 and 0.53 mg/L, and their inhibitory effects were obvious. ECso of flusilazole and pyrimethanil were 1.66 and 1.91 mg/L, and the effects were good. ECso of mancozeb and kresoxim-methyl were 25.82 and 929.78 rag/L, their virulence indexes were lower and the inhibitory effects were worse. The inhibitory effect of 72 h was similar to that of 48 h. Therefore, the inhibition effect of iprodione on Alternaria alternate was the best among the seven fungicides, when the experiment concentration was 50 mg/L, the inhibition rate was 95%.

关 键 词：玉米大斑病 链格孢菌 毒力测定 异菌脲 室内试验 病原菌分离鉴定 

分 类 号：S435.131[农业科学—农业昆虫与害虫防治]