外源性胰岛素对电化学发光免疫分析法检测胰岛素的影响  

Interference of Exogenous Insulin on Insulin Detection Test by Electrochemiluminescence Immunoassay

作　　者：张玫[1] 周君[1] 杨礼丹 桂雪梅[1] 黄俊[1] 罗开宏 黄亨建[1] ZHANG Mei;ZHOU Jun;YANG Li-dan;GUI Xue-mei;HUANG Jun;LUO Kai-hong;HUANG Heng-jian(Department of Laboratory Medicine,West China Hospital,Sichuan University,Chengdu 610041,China)

机构地区：[1]四川大学华西医院实验医学科,成都610041

出　　处：《四川大学学报（医学版）》2018年第6期924-928,共5页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金青年项目(No.81501800);四川省科技厅项目(No.2017SZ0063;No.2014SZ0170)资助

摘　　要：目的探讨外源性胰岛素对电化学发光免疫分析法检测胰岛素的影响。方法采用电化学发光免疫分析法测定血清胰岛素水平,根据美国临床实验室标准化协会标准文件EP7-A2的要求,采用配对差异实验分别评价8种外源性胰岛素对高、低浓度血清胰岛素测定的影响。如具有显著影响,用剂量效应实验评价确定外源性胰岛素浓度与干扰程度间的关系。结果甘舒霖N○R、甘舒霖R○R、优泌林R○R、诺和灵R○R、来得时○R在干扰浓度≤250μU/mL时,对高、低浓度胰岛素测定的影响均为线性正干扰;诺和平○R在干扰物浓度≤250μU/mL时,对高浓度胰岛素的影响为线性负干扰,对低浓度胰岛素无干扰,优泌乐○R和诺和锐○R对胰岛素检测无干扰。结论不同外源性胰岛素对电化学发光免疫分析法检测血清胰岛素的影响不同,临床上应注意由此导致的检测误差。Objective To explore the interference of exogenous insulin therapy on insulin detection test by electrochemical luminescence immunoassay （ECLIA）. Methods Insulin level was determined by ECLIA. According to the requirements of EP7-A2 of American Society for Clinical Laboratory Standards Institute Standards, paired difference experiment was conducted to evaluate the interference of 8 kinds of exogenous insulin on insulin detection, dose effect experiment was conducted to determine the relationship between exogenous insulin concentration and interference degree. Results When the interfering substance concentrations were ≤250 μU/mL, Gansulin N , Gansulin R , Humulin R ,Novolin R and Lantus all showed linear positive interference, while Levemir showed a linear negative interference in high concentrations insulin and non-interfering in low concentrations insulin, Humalog and Novo Rapid showed non-interference in insulin detection. Conclusion The use of different exogenous insulin may have different interference on insulin measurement, which need laboratorians and physicians notice to avoid misdiagnosis.

关 键 词：干扰试验 外源性胰岛素 电化学发光免疫分析法 

分 类 号：R446.6[医药卫生—诊断学]