复方克霉唑搽剂中醋酸氟轻松和克霉唑的含量测定  

Determination of Fluocinonide and Clotrimazole in Compound Clotrimazole Liniment by HPLC

作　　者：汪旭 徐颖 高中华 于洋 姜立波 熊东旭 陈晓亮[2] WANG Xu;XU Ying;GAO Zhong-hua;YU Yang;JIANG Li-bo;XIONG Dong-xu;CHEN Xiao-liang(Department of Pharmacy,Changchun Central Hospital,Changchun 130000,China;Department of Pharmacy,Haikou Peoplers Hospital,Haikou,570208,China)

机构地区：[1]长春市中心医院药学部,吉林长春130000 [2]海口市人民医院药学部,海南海口570208

出　　处：《解放军药学学报》2018年第5期427-429,共3页Pharmaceutical Journal of Chinese People's Liberation Army

摘　　要：目的建立HPLC法测定复方克霉唑搽剂中醋酸氟轻松和克霉唑的含量方法。方法应用HPLC法,以2种主要成分为指标,采用Diamonsil C18色谱柱(250 mm×4.6 mm,5μm);流动相为甲醇-水(73∶27);检测波长:240nm;流速:1.0 ml·min^(-1)。结果醋酸氟轻松和克霉唑的线性范围分别为0.003~0.021 mg·ml^(-1)(r=1.000)和0.12~0.84mg·ml^(-1)(r=1.000)。平均回收率分别为100.09%和99.99%,RSD为0.96%、0.33%。结论本方法简单、迅速、可靠。Objective To establish an HPLC method for determination of fluocinonide and clotrimazole in compound clotrimazole liniment.Methods The separation of the two components was achieved on a reversed-phase Diamonsil C18 column（250 mm ×4.6 mm,5μm）with methanol-water（73∶27）at a flow of 1.0 ml·min^（-1）.Results The linear range was 0.003-0.021 mg·ml^（-1）（r =1）and0.12-0.84 mg·ml^（-1）（r =1）,respectively.The average recovery was 100.09%（n =9,RSD=0.96%）and99.99%（n =9,RSD=0.33%）,respectively.Conclusion This method is simple,quick and reliable.

关 键 词：复方克霉唑搽剂 HPLC 醋酸氟轻松 克霉唑 

分 类 号：R927.2[医药卫生—药学]