哈茨木霉Tr1菌株分泌蛋白及Peptaibols合成酶的生物信息学分析  

Secreted Protein and Peptaibols Synthetase of Trichoerma harzianum Tr1: Bioinformatics Analysis

作　　者：许勇[1] 戴陈伟[1] 李静[1] 蔡标[1] 张爽[1] 童琳[1] 叶红[1] Xu Yong;Dai Chenwei;Li Jing;Cai Biao;Zhang Shuang;Tong Lin;Ye Hong(Anhui Academy of Medical Science,Hefei 230061)

机构地区：[1]安徽省医学科学研究院,合肥230061

出　　处：《中国农学通报》2018年第23期46-52,共7页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金"鸡Ii载体介导抗原靶向进入MHCI交叉递呈的作用机理"(31372417);安徽省"十三五"医疗卫生重点专科"病原微生物实验室"(皖卫科教[2017]30号);2018年度安徽省卫生计生委科研计划项目"虫荧光素酶的定向进化研究"(2018YK006);安徽省卫生计生委中医药科研课题"皖贝母内生真菌分离与鉴定"(2014zy46)

摘　　要：研究旨在应用生物信息学方法分析生防菌哈茨木霉的分泌蛋白及Peptaibols合成酶。本研究以哈茨木霉Tr1菌株的11264条假定蛋白质序列为对象,利用Signal P、Protparam和Swissmodel等生物信息学工具预测出分泌蛋白,分析其信号肽特征,从中筛选可能与其生防机制有直接联系的分泌蛋白和Peptaibols合成酶,并对这些蛋白质的性质和结构功能进行分析。结果表明:哈茨木霉Tr1菌株含有403个分泌蛋白,其中假定蛋白质PNP53160和PNP56908可能起到纤维素酶和几丁质酶的作用。分泌蛋白的信号肽的中间区域富含亮氨酸和丙氨酸,C端属于A-X-A类型。假定蛋白PNP58822中的16862~20750 aa片段可能起到Peptaibols合成酶作用,该蛋白主要包括了乙酰辅酶A合成酶Ⅰ、Taq A的CT结构域和脂肽合成酶亚基Ⅲ等功能区域。本研究应用生物信息学方法分析了403个分泌蛋白及其信号肽特征,筛选出了可能与哈茨木霉生防机制相关的蛋白质,并预测了其性质、结构和功能,为进一步研究哈茨木霉的生防机制提供理论基础。The paper aims to analyze the secreted proteins and Peptamots symnetase of Trtcnoerma uarztauttHt by bioinformatics methods. Using the 11264 hypothetical proteins of Trichoerma harzianum Trl as the objects of this study, we applied the bioinformatics methods such as SignalP, Protparam and Swissmodel to predict the secreted proteins, and analyzed their signal peptides, in order to select the secreted proteins and Peptaibols synthetase associating with the biocontrol mechanism, and analyzed the properties, structures and functions of these proteins. The results showed that： a total of 403 secreted proteins were found in Trichoerma harzianumTrl, thereinto PNP53160 and PNP56908 might act as cellulase and chitinase respectively; the intermediate region of signal peptide was rich in leucine and alanine, while the C terminals of signal peptides belonged to A-X-A type; the 16862-20750 aa of PNP58822 which contained 5 kinds of functional regions including acetyl coenzyme A synthetase I, CT domain of TaqA, and surfactin synthetase subunit IiI and so on, probably acted as Peptaibols synthetase. In this study, 403 secreted proteins and their signal peptides are analyzed by bioinformatics methods, and the characteristics, structures and functions of the proteins which are probably associated with the biocontrol mechanism of Trichoerma harzianum are predicted, so as to provide a fundamental basis for further study on its biocontrol mechanism.

关 键 词：哈茨木霉 分泌蛋白 生物信息学 信号肽 Peptaibols合成酶 

分 类 号：S763.306.4[农业科学—森林保护学]